Stem Cells and Development
| Autor: | Souza, Cleide dos Santos, Chicaybam, Leonardo, Bonamino, Martin Hernán, Bahia, Marcus Vinicius, Costa, Silvia Lima, Borges, Helena L., Rehen, Stevens K. |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Zdroj: | Repositório Institucional da UFBA Universidade Federal da Bahia (UFBA) instacron:UFBA |
| DOI: | 10.1089/scd.2010.0446 |
| Popis: | Texto completo: acesso restrito. p. 1711-1720 Submitted by Edileide Reis (leyde-landy@hotmail.com) on 2014-03-13T17:03:21Z No. of bitstreams: 1 Cleide S. Souza.pdf: 801326 bytes, checksum: c5b0023557e1956e9368e5601b957064 (MD5) Approved for entry into archive by Patricia Barroso (pbarroso@ufba.br) on 2014-03-13T18:24:47Z (GMT) No. of bitstreams: 1 Cleide S. Souza.pdf: 801326 bytes, checksum: c5b0023557e1956e9368e5601b957064 (MD5) Made available in DSpace on 2014-03-13T18:24:48Z (GMT). No. of bitstreams: 1 Cleide S. Souza.pdf: 801326 bytes, checksum: c5b0023557e1956e9368e5601b957064 (MD5) Previous issue date: 2011 Flavonoids have key functions in the regulation of multiple cellular processes; however, their effects have been poorly examined in pluripotent stem cells. Here, we tested the hypothesis that neurogenesis induced by all-trans retinoic acid (RA) is enhanced by agathisflavone (FAB, Caesalpinia pyramidalis Tull). Mouse embryonic stem (mES) cells and induced pluripotent stem (miPS) cells growing as embryoid bodies (EBs) for 4 days were treated with FAB (60 μM) and/or RA (2 μM) for additional 4 days. FAB did not interfere with the EB mitotic rate of mES cells, as evidenced by similar percentages of mitotic figures labeled by phospho-histone H3 in control (3.4%±0.4%) and FAB-treated groups (3.5%±1.1%). Nevertheless, the biflavonoid reduced cell death in both control and RA-treated EBs from mES cells by almost 2-fold compared with untreated EBs. FAB was unable, by itself, to induce neuronal differentiation in EBs after 4 days of treatment. On the other hand, FAB enhanced neuronal differentiation induced by RA in both EBs of mES and miPS. FAB increased the percentage of nestin-labeled cells by 2.7-fold (mES) and 2.4 (miPS) and β-tubulin III–positive cells by 2-fold (mES) and 2.7 (miPS) in comparison to RA-treated EBs only. FAB increased the expression of RA receptors α and β in mES EBs, suggesting that the availability of RA receptors is limiting RA-induced neurogenesis in pluripotent stem cells. This is the first report to describe that naturally occurring biflavonoids regulate apoptosis and neuronal differentiation in pluripotent stem cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|