EVALUATION OF THE FREEZABILITY OF THE BOVINE EPIDIDYMIS TAIL SPERM WITH THE ADDITION OF ANTIOXIDANTS

Autor: Cruz, Gabriela Passamani da, Zanfrilli dos Santos, Ana Paula, Freitas Guaitolini, Carlos Renato de, Denck Tramontin, Marcio Luiz, Rigoto, Renata Patricia, Crespilho, Andre Maciel, Freitas Dell'Aqua, Camila de Paula [UNESP], Mello Martins, Maria Isabel, Marques, Ana Beatriz, Tsunokawa Hidalgo, Myrian Megumy, Oliveira Sestari, Danielle Andressa, Magalhales, Ricardo, Dias Maziero, Rosiara Rosaria
Přispěvatelé: Univ Paranaense, Univ Santo Amaro, Universidade Estadual Paulista (Unesp), Universidade Estadual de Londrina (UEL)
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: Web of Science
Repositório Institucional da UNESP
Universidade Estadual Paulista (UNESP)
instacron:UNESP
Popis: Made available in DSpace on 2021-06-25T15:01:01Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-03-01 BACKGROUND: The cryopreservation and recovery of epididymis tail sperm is an important biotechnology dependent on the composition of the freezing medium. OBJETIVE: To evaluate the effect of melatonin, added to commercial freezing medium extender, on the kinetics and viability of bovine epididymis tail sperm. MATERIAL AND METHODS: Five routines were performed, each consisting of eight epididymis and the structures were sliced onto a glass plate containing a commercial diluting medium for Botubov (R). The samples were divided into four groups, with 80 x 10(6) spermatozoa per mL. Group 1: samples diluted in Botubov (R). Group 2: samples centrifuged (600 g, 10 min), and the pellet re-suspended in Botubov (R). Group 3, samples diluted in Botubov (R) containing 100 pM melatonin. Group 4: samples centrifuged (600 g, 10 min) and the pellet resuspended in Botubov (R) with 100 pM melatonin. The samples were transferred to 0.5 mL straws at 40 x 10(6) viable spermatozoa, stabilized at 5 degrees C for 4 h, transferred to liquid nitrogen vapour for 20 min, dipped in liquid nitrogen and stored in a cryogenic cylinder. After thawing (46 degrees C, 15 s), sperm kinetics and viability parameters were evaluated. RESULTS: There was no difference in the parameters of total motility (MT, %), progressive motility (MP, %), progressive linear velocity (VSL, mu m/s), curvilinear velocity (VCL, mu m/s), linearity (LIN, %), spermatozoa with rapid movement (RAP, %) and level of intact plasma membranes and acrosome (IPMA, %) among the groups studied. However, a difference was observed between the routines performed. CONCLUSION: The protocol for freezing bovine epididymis tail sperm is applicable; however, there is an influence of the epididymis used, for the best efficacy of this biotechnology. Univ Paranaense, Umuarama, PR, Brazil Univ Santo Amaro, Sao Paulo, SP, Brazil FMVZ UNESP, Dept Radiol & Anim Reprod, Botucatu, SP, Brazil Univ Estadual Londrina, UEL, Londrina, Parana, Brazil FMVZ UNESP, Dept Radiol & Anim Reprod, Botucatu, SP, Brazil
Databáze: OpenAIRE
Externí odkaz: