Antibacterial evaluation of Jambu (Spilanthes acmella) plant extract in Staphylococcus spp. in bovine milk samples

Autor: Chagas, Mois??s Martins, https://orcid.org/0000-0003-1520-2644
Přispěvatelé: Tonin, Alexandre Alberto, Santos, Felipe Faccini dos, Sousa, Rejane dos Santos, Costa Neto, Pedro de Queiroz, Tochetto, Camila
Jazyk: portugalština
Rok vydání: 2020
Předmět:
Zdroj: Biblioteca Digital de Teses e Dissertações da UFAM
Universidade Federal do Amazonas (UFAM)
instacron:UFAM
Popis: Submitted by Mois??s Chagas (moises.sbz@gmail.com) on 2020-12-11T14:02:23Z No. of bitstreams: 3 Disserta????o - Mois??s.pdf: 1868392 bytes, checksum: 14313c0c057b498e28358f268ea88a89 (MD5) CartaEncaminhamentoAutodep??sito.pdf: 32552 bytes, checksum: 50ada9d3f53a9b2faef8dbb345e0f743 (MD5) SEI_UFAM - 0284659 - Ata (1).pdf: 130624 bytes, checksum: 4f85f9e90187724b199dde224dae7efe (MD5) Approved for entry into archive by PPGCAN Ci??ncia Animal (ppgcan.ufam@gmail.com) on 2020-12-11T19:19:52Z (GMT) No. of bitstreams: 3 Disserta????o - Mois??s.pdf: 1868392 bytes, checksum: 14313c0c057b498e28358f268ea88a89 (MD5) CartaEncaminhamentoAutodep??sito.pdf: 32552 bytes, checksum: 50ada9d3f53a9b2faef8dbb345e0f743 (MD5) SEI_UFAM - 0284659 - Ata (1).pdf: 130624 bytes, checksum: 4f85f9e90187724b199dde224dae7efe (MD5) Approved for entry into archive by Divis??o de Documenta????o/BC Biblioteca Central (ddbc@ufam.edu.br) on 2020-12-11T19:53:59Z (GMT) No. of bitstreams: 3 Disserta????o - Mois??s.pdf: 1868392 bytes, checksum: 14313c0c057b498e28358f268ea88a89 (MD5) CartaEncaminhamentoAutodep??sito.pdf: 32552 bytes, checksum: 50ada9d3f53a9b2faef8dbb345e0f743 (MD5) SEI_UFAM - 0284659 - Ata (1).pdf: 130624 bytes, checksum: 4f85f9e90187724b199dde224dae7efe (MD5) Made available in DSpace on 2020-12-11T19:53:59Z (GMT). No. of bitstreams: 3 Disserta????o - Mois??s.pdf: 1868392 bytes, checksum: 14313c0c057b498e28358f268ea88a89 (MD5) CartaEncaminhamentoAutodep??sito.pdf: 32552 bytes, checksum: 50ada9d3f53a9b2faef8dbb345e0f743 (MD5) SEI_UFAM - 0284659 - Ata (1).pdf: 130624 bytes, checksum: 4f85f9e90187724b199dde224dae7efe (MD5) Previous issue date: 2020-08-20 Funda????o de Amparo ?? Pesquisa do Estado do Amazonas - FAPEAM In Brazil, the genus Staphylococcus spp is considered as the main causal agent of bovine mastitis. Several studies indicate that both S. aureus bacteria and coagulase-negative staphylococci, in addition to being the most frequent and found in mastitis, have an increasing pattern of resistance to antimicrobials, which are opportunistic, leading to intramammary infections and decreasing milk quality. One of the alternatives found to help reduce costs and, mainly, to minimize the processes of microbiological resistance is the use of phytotherapeutic agents (or derivatives thereof) as therapeutic adjuvant agents. Among these potential therapeutic alternatives, we find Jambu (Spilanthes acmella), a plant rich in bioactive isobutyl amides. Due to the presence of this substance, the plant has traditional applications in pharmaceutical products, food, and products for health and personal care. Thus, the objective of this work was to evaluate the antibacterial effects of the Jambu plant extract in isolates of Staphylococcus spp. from bovine milk samples. For this purpose, 60 milk samples were collected from cows from municipalities in the interior of the State of Amazonas. These samples were made for the detection, counting, and confirmation of Staphylococcus spp and, from these, the minimum inhibitory concentration test of the Jambu plant extract. As a result, Of the 60 milk samples, it was possible to obtain colonies characteristic of Staphylococcus spp. of 56 (93.3%) samples. Of these, 56 strains were obtained, of which 19 (33.9%) were characterized as Staphylococcus aureus and 37 (66.1%) as Staphylococcus spp. All 56 strains showed some level of sensitivity to the Jambu plant extract, from the initial dilution (20% concentration) to the final dilution (0.039%). In our study, we obtained a high percentage of isolation (93.3%) of Staphylococcus spp from the samples, with 33.9% representing Staphylococcus aureus and 66.1% of coagulase-negative staphylococcus. In this scenario, we found that the Jambu plant extract inhibited the growth of Staphylococcus spp. isolated from bovine milk samples, in all tested concentrations, except the last one (at 0.0195%). For isolates with a lower inhibitory concentration, the effects, for the most part, behaved as bacteriostatic, whereas for isolates with a higher inhibitory concentration the effect was similar to the bactericide. We believe that these differences in effects may be linked to different levels of sensitivity of the isolates. No Brasil, o g??nero Staphylococcus spp ?? considerado como o principal agente causal da mastite bovina. Diversos estudos apontam que tanto a bact??ria S. aureus, como os estafilococos de coagulase-negativa al??m de serem os mais frequentes e encontrados na mastite, apresentam aumento crescente no padr??o de resist??ncia a antimicrobianos, de caracter??stica oportunista, acarretam em infec????es intramam??rias e diminuem a qualidade do leite. Uma das alternativas encontradas para auxiliar na redu????o dos custos e, principalmente, para minimizar os processos de resist??ncia microbiol??gica ?? a utiliza????o de agentes fitoter??picos (ou derivados destes) como agentes adjuvantes terap??uticos. Dentre estas potencias alternativas terap??uticas encontramos o Jambu (Spilanthes acmella), planta rica em isobutilamidas bioativas. Devido ?? presen??a desta subst??ncia, a planta possui aplica????o tradicional em produtos farmac??uticos, alimentos e produtos para a sa??de e cuidados pessoais. Desta forma, o objetivo deste trabalho foi avaliar os efeitos antibacterianos do extrato vegetal de Jambu em isolados de Staphylococcus spp. provenientes de amostras de leite bovino. Para este prop??sito foram coletadas 60 amostras de leite de vacas de munic??pios do interior do Estado do Amazonas. Estas amostras foram feitas a detec????o, contagem e confirma????o de Staphylococcus spp e, a partir destas, o teste de concentra????o inibit??ria m??nima do extrato vegetal de Jambu. Como resultados, Das 60 amostras de leite, foi poss??vel obter col??nias caracter??sticas de Staphylococcus spp. de 56 (93,3%) amostras. Destas, foram obtidas 56 cepas, sendo 19 (33,9%) caracterizadas como Staphylococcus aureus e 37 (66,1%) como Staphylococcus spp. Todas as 56 cepas apresentaram algum n??vel de sensibilidade ao extrato vegetal de Jambu, desde a dilui????o inicial (concentra????o de 20%) at?? a dilui????o final (0,039%). Em nosso estudo obtivemos uma alta percentagem de isolamento (93,3%) de Staphylococcus spp das amostras, com 33,9% representando Staphylococcus aureus e 66,1% de estafilococus coagulase-negativa. Neste cen??rio, verificamos que o extrato vegetal de Jambu provocou inibi????o no crescimento de Staphylococcus spp. isolados de amostras de leite bovina, em todas as concentra????es testadas, exceto a ??ltima (a 0,0195%). Para os isolados com concentra????o inibit??ria menor os efeitos, em sua maioria, se comportaram como bacteriost??tico, ao passo que para isolados com concentra????o inibit??ria maiores o efeito foi similar ao bactericida. Acreditamos que essas diferen??as em efeitos possam estar ligadas a diferentes n??veis de sensibilidade dos isolados. Em car??cter pessoal, apenas na quest??o de g??nero, pois envolve marca????o e n??o marca????o, o que foi um pouco confuso. ?? uma pesquisa importante, pois se trabalha com um produto da regi??o Amaz??nica, neste caso o jambu para a pesquisa atrav??s do conhecimento de suas propriedades anti-microbianas.
Databáze: OpenAIRE