Investiga????o de genes de virul??ncia associados ao processo invasivo de diferentes cepas de Shigella spp. de origem cl??nica

Autor: Rezende, Alan de Oliveira
Přispěvatelé: Orlandi, Patr??cia Puccinelli, Matos, Najla Benevides, Castro, Diogo Pereira de Castro
Jazyk: portugalština
Rok vydání: 2016
Předmět:
Zdroj: Biblioteca Digital de Teses e Dissertações da UFAM
Universidade Federal do Amazonas (UFAM)
instacron:UFAM
Popis: Submitted by Alan Rezende (alanoliveirarezende@gmail.com) on 2021-08-26T18:18:30Z No. of bitstreams: 4 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Ata de defesa_Alan de Oliveira Rezende.pdf: 204597 bytes, checksum: 4c352d37041e69baf019e2b0e5b67fc0 (MD5) Disserta????o_Alan de Oliveira Rezende.pdf: 2215337 bytes, checksum: 5705f66e500fff4c1b176b0114d5ce0b (MD5) Carta Encaminhamento Autodep??sito.pdf: 137222 bytes, checksum: bf413ad62d71dedac785b5a7ff9989f4 (MD5) Approved for entry into archive by PPGBIOTEC Biotecnologia (ppgbiotecsecretaria@ufam.edu.br) on 2021-08-26T22:56:43Z (GMT) No. of bitstreams: 4 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Ata de defesa_Alan de Oliveira Rezende.pdf: 204597 bytes, checksum: 4c352d37041e69baf019e2b0e5b67fc0 (MD5) Disserta????o_Alan de Oliveira Rezende.pdf: 2215337 bytes, checksum: 5705f66e500fff4c1b176b0114d5ce0b (MD5) Carta Encaminhamento Autodep??sito.pdf: 137222 bytes, checksum: bf413ad62d71dedac785b5a7ff9989f4 (MD5) Rejected by Divis??o de Documenta????o/BC Biblioteca Central (ddbc@ufam.edu.br), reason: A Ficha catalogr??fica precisa de ajuste: a) No t??tulo utilize letras mai??sculas somente para o in??cio do t??tulo, siglas ou nomes pr??prios. Portanto, "Investiga????o de genes de virul??ncia associados ao processo invasivo de diferentes cepas de Shigella spp. de origem cl??nica" b) Na palavra-chave n.5, n??o inserir ponto no lugar de uma palavra. Se n??o h?? termo, deixe-o em branco. on 2021-08-27T01:07:14Z (GMT) Submitted by Alan Rezende (alanoliveirarezende@gmail.com) on 2021-08-27T12:16:01Z No. of bitstreams: 4 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Ata de defesa_Alan de Oliveira Rezende.pdf: 204597 bytes, checksum: 4c352d37041e69baf019e2b0e5b67fc0 (MD5) Carta Encaminhamento Autodep??sito.pdf: 137222 bytes, checksum: bf413ad62d71dedac785b5a7ff9989f4 (MD5) Disserta????o_Alan de Oliveira Rezende.pdf: 1427276 bytes, checksum: 2c6834ebd49c1bfbcd0c77cac3f8f14f (MD5) Approved for entry into archive by PPGBIOTEC Biotecnologia (ppgbiotecsecretaria@ufam.edu.br) on 2021-08-27T14:16:08Z (GMT) No. of bitstreams: 4 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Ata de defesa_Alan de Oliveira Rezende.pdf: 204597 bytes, checksum: 4c352d37041e69baf019e2b0e5b67fc0 (MD5) Carta Encaminhamento Autodep??sito.pdf: 137222 bytes, checksum: bf413ad62d71dedac785b5a7ff9989f4 (MD5) Disserta????o_Alan de Oliveira Rezende.pdf: 1427276 bytes, checksum: 2c6834ebd49c1bfbcd0c77cac3f8f14f (MD5) Approved for entry into archive by Divis??o de Documenta????o/BC Biblioteca Central (ddbc@ufam.edu.br) on 2021-08-27T18:35:18Z (GMT) No. of bitstreams: 4 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Ata de defesa_Alan de Oliveira Rezende.pdf: 204597 bytes, checksum: 4c352d37041e69baf019e2b0e5b67fc0 (MD5) Carta Encaminhamento Autodep??sito.pdf: 137222 bytes, checksum: bf413ad62d71dedac785b5a7ff9989f4 (MD5) Disserta????o_Alan de Oliveira Rezende.pdf: 1427276 bytes, checksum: 2c6834ebd49c1bfbcd0c77cac3f8f14f (MD5) Made available in DSpace on 2021-08-27T18:35:18Z (GMT). No. of bitstreams: 4 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Ata de defesa_Alan de Oliveira Rezende.pdf: 204597 bytes, checksum: 4c352d37041e69baf019e2b0e5b67fc0 (MD5) Carta Encaminhamento Autodep??sito.pdf: 137222 bytes, checksum: bf413ad62d71dedac785b5a7ff9989f4 (MD5) Disserta????o_Alan de Oliveira Rezende.pdf: 1427276 bytes, checksum: 2c6834ebd49c1bfbcd0c77cac3f8f14f (MD5) Previous issue date: 2016-11-16 CNPQ - Conselho Nacional de Desenvolvimento Cient??fico e Tecnol??gico Enterobacteria are the main cause of dysentery worldwide. From this family, Shigella is noteworthy for being the causative agent of shigellosis, an enteric disease that annually affects about 165 million people, leading to death over one million, with children under five years of age being the main affected age group. Its transmission occurs through the fecal-oral route through ingestion of contaminated food or water, or contact between people, being more effective in places where basic sanitation is precarious or non-existent. These bacteria have the potential to escape the immune system, invading and destroying the cells that line the large intestine, where they cause ulcerations in the mucosa and diarrhea with blood and mucus. In addition to diarrhea, the infection triggers symptoms such as fever, abdominal cramps, and rectal pain, usually the uncomplicated recovery of these patients occurs within seven days. From 2007 to 2009, the research group of the Laboratory for Diagnosis and Control of Infectious Diseases in the Amazon carried out an epidemiological study evaluating the pathogens that cause diarrhea in children treated in public hospitals in Manaus - AM. Among the isolated pathogens, Shigella was identified as the fifth major causative agent of diarrhea among the cases found. Molecular studies performed with this pathogen identified variations between the isolated strains in the presence of key virulence genes for the activation of the invasion and subsequent clinical manifestation. As all strains of Shigella were isolated from cases of diarrhea, we asked ourselves about the relationship of these genes with in vitro invasion. Given the above, we characterized genus, species and virulence genes of Shigella with the use of Polymerase Chain Reaction (PCR), we identified the antibiotic resistance profile, we also analyzed the invasiveness of these different strains of clinical origin, by means of in vitro tests in phagocytic and non-phagocytic cells, in a qualitative and quantitative way. The results evidenced a strong association with the presence of virulence genes and cell invasion of Shigella strains of clinical origin. Keywords: Shigella; virulence genes; Cell Invasion; Dysentery As enterobact??rias s??o as principais causas de disenteria em todo o mundo. Desta fam??lia, a Shigella merece destaque por ser o agente causativo da shigelose, doen??a ent??rica que atinge anualmente cerca de 165 milh??es de pessoas, levando a ??bito mais de um milh??o, sendo crian??as menores de cinco anos de idade a principal faixa et??ria afetada. Sua transmiss??o ocorre pela rota fecal-oral atrav??s da ingest??o de alimentos ou ??gua contaminados, ou contato entre pessoas, sendo mais efetivas em locais onde o saneamento b??sico ?? prec??rio ou inexistente. Essas bact??rias possuem o potencial de realizar o escape do sistema imune, invadindo e destruindo as c??lulas que revestem o intestino grosso, onde causam ulcera????es na mucosa e diarreia com sangue e muco. Al??m da diarreia, a infec????o desencadeia sintomas como febre, c??lica abdominal e dor retal, geralmente a recupera????o sem complica????es desses pacientes ocorre dentro de sete dias. Durante os anos de 2007 a 2009, o grupo de pesquisa do Laborat??rio de Diagn??stico e Controle de Doen??as Infecciosas na Amaz??nia realizou um estudo epidemiol??gico avaliando os pat??genos causadores de diarreia em crian??as atendidas em hospitais p??blicos de Manaus - AM. Dentre os pat??genos isolados, a Shigella foi identificada como o quinto maior agente causador de diarreia entre os casos encontrados. Estudos moleculares realizados com este pat??geno identificaram varia????es entre as cepas isoladas na presen??a de genes de virul??ncia chaves para a ativa????o da invas??o e posterior manifesta????o cl??nica. Como todas as cepas de Shigella foram isoladas de casos de diarreia, nos questionamos sobre a rela????o desses genes com a invas??o in vitro. Diante do exposto, foi utilizado caracterizamos g??nero, esp??cies e genes de virul??ncia de Shigella com a utiliza????o da Rea????o em Cadeia da Polimerase (PCR), identificamos o perfil de resist??ncia a antibi??ticos, tamb??m analisamos a capacidade invasiva dessas diferentes cepas de origem cl??nica, por meio dos testes in vitro em c??lulas fagoc??ticas e n??o fagoc??ticas, de forma qualitativa e quantitativa. Os resultados evidenciaram uma forte associa????o com a presen??a dos genes de virul??ncia e a invas??o celular das cepas de Shigella de origem cl??nica. Palavras-chave: Shigella; Genes de virul??ncia; Invas??o Celular; Disenteria.
Databáze: OpenAIRE
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