Phenotypic, cytogenetic and molecular genetic analysis in black-horned capuchin, Sapajus nigritus (Platyrrhini, Cebidae), from areas of Rio de Janeiro and S?o Paulo states, Brazil

Autor: Penedo, Diego Mattos
Přispěvatelé: Nogueira, Denise Monnerat, Armada, Jorge Lu?s Azevedo de, Verona, Carlos Eduardo da Silva, Silva, H?lio Ricardo da, Geise, Lena, Nieves, Mariela
Jazyk: portugalština
Rok vydání: 2020
Předmět:
Zdroj: Biblioteca Digital de Teses e Dissertações da UFRRJ
Universidade Federal Rural do Rio de Janeiro (UFRRJ)
instacron:UFRRJ
Popis: Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2022-12-07T20:44:29Z No. of bitstreams: 1 2020 - Diego Mattos Penedo.pdf: 3606622 bytes, checksum: 79a44b9541512d3f705fbbf16fac11c2 (MD5) Made available in DSpace on 2022-12-07T20:44:29Z (GMT). No. of bitstreams: 1 2020 - Diego Mattos Penedo.pdf: 3606622 bytes, checksum: 79a44b9541512d3f705fbbf16fac11c2 (MD5) Previous issue date: 2020-07-30 CAPES - Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior The black-horned capuchin, Sapajus nigritus, is a Neotropical primate with a wide distribution in the Atlantic Forest, from southeast Brazil to Misiones province, Argentina. The species presents phenotypic and genetic diversity, often associated with biogeographic patterns. The coat coloration varies with intraspecific differences, with transition in the predominance from black to brown pattern, in the south-north distribution. Cytogenetic analysis in populations from Argentina showed a specific polymorphism in the chromosome pair 11. While in most Sapajus? species this pair is the largest acrocentric, with a terminal extracentromeric heterochromatic (HC) block, in S. nigritus, in these populations, the total deletion of HC is described. However, the terminal HC block has already been demonstrated in specimens from Rio de Janeiro (RJ). In addition to the chromosomal divergence, phylogenetic data demonstrate paraphyly among samples from RJ and other localities along the distribution. These variations may correspond to a pattern of phylogeographic discontinuity described in Atlantic Forest at S?o Paulo (SP), associated to forest refuges occurring in Pleistocene. The objective of this study was to analyze the phenotypic and genetic diversity of S. nigritus in SP and RJ, investigating the coat patterns, the distribution of HC in the par 11 and the molecular diversity in the state of RJ, comparing it with other localities. Individuals from five populations in the RJ state were analyzed: Ilha da Marambaia (IM), Reserva Biol?gica de Po?o das Antas (RBPA), Parque Nacional do Itatiaia (PNI), da Tijuca (PNT) and da Serra dos ?rg?os (PARNASO); as well as ex situ specimens from Guaratiba/RJ (Gua) and cities of S?o Paulo: capital (cSP), S?o Jos? dos Campos (SJC), S?o Sebasti?o (SS), Paraibuna (PB) and Campos do Jord?o (CJ). Specimens of S. nigritus at the Museu Nacional do Rio de Janeiro (MNRJ), from Angra dos Reis, Itatiaia, Paraty and Teres?polis, were included in the phenotypic analysis. Coloration of the back, belly, limbs, tail and face were evaluated. The presence and distribution of HC were analyzed using classical and molecular cytogenetic techniques. The phylogenetic relationships were inferred based on the mitochondrial genes cytochrome c oxidase I, II and cytochrome b, including sequences available in GenBank?. In 49 individuals captured, six ex situ and eight MNRJ specimens were identified brown (n=37) and black (n=26) coat patterns, with intrapopulation variation, predominating black to the west (PNI and MNRJ) and brown to the east (IM and PNT). The association of this finding with the predominance of the black pattern described in an SP population suggests a limit on the black-brown variation in the SP-RJ distribution. The pair 11 with the deletion of HC was observed in the samples to the east (IM, Gua, PNT, PARNASO and RBPA), while to the west both patterns were identified, without (cSP, CJ and SS) and with (SJC, PB and PNI) HC block. Molecular divergence was observed between the samples from RJ and those from SP, PR and Argentina, including the PNI, forming paraphyletic clades. The intraspecific diversity observed in S. nigritus may represent evolutionary significant units (ESU) and confirm the sampled region as a phylogeographic discontinuity point for the species. O macaco-prego-preto, Sapajus nigritus, ? um primata neotropical com ampla distribui??o na Mata Atl?ntica, nos estados do sudeste e sul do Brasil e na prov?ncia de Misiones, Argentina. A esp?cie apresenta diversidade fenot?pica e gen?tica, muitas vezes associadas a padr?es biogeogr?ficos. A colora??o da pelagem varia de forma intraespec?fica, sendo sugerida transi??o na predomin?ncia do padr?o preto ao marrom, no sentido sul-norte da distribui??o. An?lises citogen?ticas em popula??es da Argentina demonstram polimorfismo espec?fico no par cromoss?mico 11. Enquanto na maioria das esp?cies de Sapajus esse par ? o maior acroc?ntrico, com grande bloco de heterocromatina extra-centrom?rica (HC) terminal, em S. nigritus, nessas popula??es, ? descrita dele??o total da HC. No entanto, o grande bloco de HC terminal j? foi demonstrado em exemplares do Rio de Janeiro (RJ). Al?m da diverg?ncia cromoss?mica, dados filogen?ticos demonstram parafilia entre amostras do RJ e de outras localidades ao longo da distribui??o. Essas varia??es podem corresponder a um padr?o de descontinuidade filogeogr?fica descrito na regi?o de Mata Atl?ntica em S?o Paulo (SP), associado a ref?gios florestais ocorridos no Pleistoceno. O objetivo deste estudo foi analisar a diversidade fenot?pica e gen?tica de S. nigritus em SP e RJ, averiguando os padr?es de pelagem, a distribui??o da HC no par 11 e a diversidade molecular no estado do RJ, comparando-a com outras localidades. Foram analisados indiv?duos de cinco popula??es do estado do RJ: Ilha da Marambaia (IM), Reserva Biol?gica de Po?o das Antas (RBPA), Parque Nacional do Itatiaia (PNI), da Tijuca (PNT) e da Serra dos ?rg?os (PARNASO); al?m de exemplares ex situ de Guaratiba/RJ (Gua) e de cidades de S?o Paulo: capital (cSP), S?o Jos? dos Campos (SJC), S?o Sebasti?o (SS), Paraibuna (PB) e Campos do Jord?o (CJ). Esp?cimes de S. nigritus do Museu Nacional do Rio Janeiro (MNRJ), provenientes de Angra dos Reis, Itatiaia, Paraty e Teres?polis foram inclu?dos nas an?lises fenot?picas. Foi avaliada a colora??o do dorso, ventre, membros, cauda e face. A presen?a e a distribui??o de HC foram analisadas com t?cnicas de citogen?tica cl?ssica e molecular. As rela??es filogen?ticas foram inferidas com base nos genes mitocondriais citocromo c oxidase I, II e citocromo b, incluindo sequ?ncias dispon?veis no GenBank?. Em 49 indiv?duos capturados, seis ex situ e oito exemplares do MNRJ foram identificados os padr?es de pelagem marrom (n=37) e preto (n=26), com varia??o intrapopulacional, predominando o preto ? oeste (PNI e MNRJ) e marrom ? leste (IM e PNT). A associa??o desse achado ? predomin?ncia do padr?o preto descrita em uma popula??o de SP sugere um limite na varia??o preto-marrom na distribui??o SP-RJ. O par 11 com a dele??o da HC foi observado nas amostras ? leste (IM, Gua, PNT, PARNASO e RBPA), enquanto ? oeste ambos os padr?es foram identificados, sem (cSP, CJ e SS) e com (SJC, PB e PNI) bloco de HC. Foi observada diverg?ncia molecular entre as amostras do RJ e aquelas de SP, PR e Argentina, incluindo o PNI, formando clados parafil?ticos. A diversidade intraespec?fica observada em S. nigritus pode representar unidades evolutivamente significativas (UES) e confirmar a regi?o amostrada como um ponto de descontinuidade filogeogr?fica para a esp?cie.
Databáze: OpenAIRE
Externí odkaz: