Analytical and Bioanalytical Chemistry
| Autor: | Jesus, Joilson Ramos, Carvalho, Kellyanne dos Anjos, Fonseca, Rosana Anita da Silva, Oliveira, Geraldo Gileno de Sá, Melo, Stella Maria Barrouin, Neves, Neuza Maria Alcântara, Dutra, Rosa Amália Fireman |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: | |
| Zdroj: | DOI 10.1007/s00216-011-5136-7 Repositório Institucional da UFBA Universidade Federal da Bahia (UFBA) instacron:UFBA |
| DOI: | 10.1007/s00216-011-5136-7 |
| Popis: | texto completo: restrito. p. 917–925 Submitted by Ana Valéria de Jesus Moura (anavaleria_131@hotmail.com) on 2012-02-09T16:41:11Z No. of bitstreams: 1 8951fulltext.pdf: 241170 bytes, checksum: 59707dfe33fe7c188ddf0ee00eb160e0 (MD5) Made available in DSpace on 2012-02-09T16:41:11Z (GMT). No. of bitstreams: 1 8951fulltext.pdf: 241170 bytes, checksum: 59707dfe33fe7c188ddf0ee00eb160e0 (MD5) Previous issue date: 2011 The American visceral leishmaniasis is an important cause of morbidity and mortality in Brazil for both humans and dogs. Attempts to make a diagnosis of this disease need to be improved, especially in endemic areas, and in the tracking and screening of asymptomatic dogs, which are their main host in urban areas. A quartz crystal microbalance immunosensor for the diagnosis of the canine visceral leishmaniasis using a recombinant antigen of Leishmania chagasi (rLci2B-NH6) was devel- oped. The rLci2B-NH6 was tightly immobilized on a quartz crystal gold electrode by self-assembled monolayer based on short-chain length thiol. The strategy was the use of the antigen-histidine tail covalently linked to glutaral-dehyde performing a Schift base which permits a major exposure of epitopes and a reduced steric hindrance. The immunosensor showed good results regarding sensitivity and reproducibility, being able to distinguish positive and negative canine serum for L. chagasi. Furthermore, the immunosensor can be reused through exposure to sodium dodecyl sulfate solution, which promotes the dissociation of antigen–antibody binding, restoring the sensor surface with immobilized biologically active antigens for further analysis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|