American Journal of Tropical Medicine and Hygiene
| Autor: | Cerqueira, Gustavo M., McBride, Alan J. A., Queiroz, Adriano, Pinto, Luciano S., Silva, Éverton F., Hartskeerl, Rudy A., Reis, Mitermayer G., Ko, Albert I., Dellagostin, Odir Antonio |
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| Jazyk: | angličtina |
| Rok vydání: | 2010 |
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| Zdroj: | Repositório Institucional da UFBA Universidade Federal da Bahia (UFBA) instacron:UFBA |
| DOI: | 10.4269/ajtmh.2010.09-0558 |
| Popis: | Texto completo: acesso restrito. p.83-87 Submitted by Suelen Reis (suziy.ellen@gmail.com) on 2013-09-11T13:14:58Z No. of bitstreams: 1 2010.09-0558.pdf: 485495 bytes, checksum: b03bac0e10bd69c6989e1ca198a5b1c1 (MD5) Approved for entry into archive by Vilma Conceição(vilmagc@ufba.br) on 2013-09-13T17:18:48Z (GMT) No. of bitstreams: 1 2010.09-0558.pdf: 485495 bytes, checksum: b03bac0e10bd69c6989e1ca198a5b1c1 (MD5) Made available in DSpace on 2013-09-13T17:18:48Z (GMT). No. of bitstreams: 1 2010.09-0558.pdf: 485495 bytes, checksum: b03bac0e10bd69c6989e1ca198a5b1c1 (MD5) Previous issue date: 2010 The purpose of this study was to perform a 16S sequence-based quality control of two Leptospira strain collections. 16S rRNA gene sequencing was used to verify two Leptospira reference collections provided by the World Health Organization and maintained at a reference laboratory for leptospirosis in Brazil. Among the 89 serovars evaluated, four conflicting strains were identified in one of the collections. Although 16S rRNA gene sequencing cannot identify Leptospira beyond the species level, it is suitable for the identification of contamination and quality control of leptospiral reference collections. This study highlights the importance of the availability of high-quality 16S rRNA sequences in public databases. In addition, it emphasizes the need for periodical verifications and quality control of Leptospira reference collections. |
| Databáze: | OpenAIRE |
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