Influence of pH and ionic strength on the antibacterial effect of hyaluronic acid/chitosan films assembled layer-by-layer

Autor: Nascimento, Vicente Franco, 1984, França, Carla Giometti, 1989, Hernández Montelongo, Jesús Jacobo, 1979, Machado, Daisy, 1981, Lancellotti, Marcelo, 1976, Cotta, Mônica Alonso, 1963, Landers, Richard, 1946, Beppu, Marisa Masumi, 1972
Přispěvatelé: UNIVERSIDADE ESTADUAL DE CAMPINAS
Rok vydání: 2018
Předmět:
Zdroj: Repositório da Produção Científica e Intelectual da Unicamp
Universidade Estadual de Campinas (UNICAMP)
instacron:UNICAMP
Popis: Agradecimentos: The authors acknowledge the support from Multi-User Lab (LAMULT) from Institute of Physics "Gleb Wataghin" at UNICAMP, which provided invalu-able access to their equipments. This work was financially supported FAPEAM, CNPq, and CAPES Abstract: Hyaluronic acid (HA)/chitosan (CHI) films were assembled by LbL using different conditions of pH and ionic strength (IS) in order to obtain stable surfaces to avoid the adhesion and proliferation of Pseudomonas aeruginosa and Staphylococcus aureus. The results showed that pH and IS were key synthesis variables for obtaining different features, such as wettability, thickness and the availability of functional groups. The antibacterial effect was more effective against S. aureus with a reduction of approximately 3, 4, and 1.5 log reduction after 4, 8 and 24 h of culture time, respectively. In the case of P. aeruginosa, the films presented a lower bacterial reduction: 1 log reduction in times evaluated. In conclusion, the results of the antibacterial effect against S. aureus after 24 h, suggest that the HA/CHI films assembled by LbL can protect several substrates, such as biomedical devices, against bacterial proliferation FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DO AMAZONAS - FAPEAM CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQ COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPES Fechado
Databáze: OpenAIRE
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