Photodynamic therapy effect on chemotaxis of macrophages and T lymphocytes activated in periodontal tissue

Autor: Evangelista, ??rika Elisabeth
Přispěvatelé: Fran??a, Cristiane Miranda, Navarro, Ricardo Scarparo, Prates, Renato Araujo
Jazyk: portugalština
Rok vydání: 2014
Předmět:
Zdroj: Biblioteca Digital de Teses e Dissertações da Uninove
Universidade Nove de Julho (UNINOVE)
instacron:UNINOVE
Popis: Submitted by Nadir Basilio (nadirsb@uninove.br) on 2016-05-19T15:16:35Z No. of bitstreams: 1 Erika Elisabeth Evangelista.pdf: 1020129 bytes, checksum: dee500eb8c5a17c94acdc64c0d81e2de (MD5) Made available in DSpace on 2016-05-19T15:16:35Z (GMT). No. of bitstreams: 1 Erika Elisabeth Evangelista.pdf: 1020129 bytes, checksum: dee500eb8c5a17c94acdc64c0d81e2de (MD5) Previous issue date: 2014-12-11 Periodontal disease is an inflammatory disease affecting the supporting tissues of the teeth, leading to loss of periodontal ligament and bone. The treatment involves scaling and root planning, and in more advanced cases there is the need for surgery and sometimes antibiotics. Photodynamic therapy is a therapeutic antimicrobial resource to aid in modulation of the inflammatory response triggered by the light. One of the main cells is the macrophage inflammatory process which acts in reciprocity with T lymphocytes. Thus the aim of this study was to evaluate the effect of photodynamic therapy in the chemotaxis of macrophages and activated T lymphocytes in the periodontal tissue. For such, patients who have undergone previous periodontal treatment with good oral hygiene and periodontal pockets residual requiring surgery were allocated. PDT was applied on the following parameters: methylene blue (50ug / ml) was applied to the bottom of the pocket and after 5 minutes via transmucosal the photosensitizer was irradiated with diode laser 660 nm, P = 100 mW, t = 90 s per point 9 J/point; energy density: 22 J/cm2, power density: 250 mW/cm2. One week after PDT, the surgical removal of the pockets was performed and samples sent for routine histology and immunohistochemical detection of activated T lymphocytes (CD45RO +) and macrophages (CD68 +). The samples were photographed and positive cells counted with the aid of ImageJ software. Data were evaluated statistically and no significant difference between groups (Mann-Whitney p = 0.9). We conclude that PDT does not interfere with chemotaxis of macrophages and activated T lymphocytes in periodontal tissue after seven days. A doen??a periodontal ?? uma doen??a inflamat??ria que afeta os tecidos de suporte dos dentes, levando ?? perda de ligamento periodontal e de osso. O tratamento consiste em raspagem e alisamento radicular e nos casos mais avan??ados h?? a necessidade de cirurgias e, ??s vezes antibioticoterapia. A terapia fotodin??mica ?? um recurso terap??utico antimicrobiano que auxilia a modula????o da resposta inflamat??ria desencadeada pela luz. Uma das principais c??lulas do processo inflamat??rio ?? o macr??fago, que age em reciprocidade com os linf??citos T. Assim o objetivo deste trabalho foi avaliar o efeito da terapia fotodin??mica na quimiotaxia de macr??fagos e linf??citos T ativados no tecido periodontal. Para tal foi realizado um estudo cl??nico transversal no qual foram alocados pacientes que passaram por tratamento periodontal pr??vio com boa higiene oral e bolsas periodontais residuais com indica????o cir??rgica. A PDT foi aplicada nos seguintes par??metros: azul de metileno (50ug/mL) aplicado no fundo da bolsa e ap??s 5 minutos foi irradiado via transmucosa com laser de diodo, 660 nm, P= 100 mW, t= 90 s por ponto, 9 J/ponto, densidade de energia: 22 J/cm2, densidade de pot??ncia: 250 mW/cm2. Uma semana ap??s a PDT foi realizada remo????o cir??rgica das bolsas e as amostras encaminhadas para processamento histol??gico de rotina e imunohistoqu??mica para detec????o de linf??citos T ativados (CD45ro+) e macr??fagos (CD68+). As amostras foram fotografadas e as c??lulas positivas contadas com aux??lio do software ImageJ. Os dados foram avaliados estatisticamente e n??o houve diferen??a significativa entre os grupos (Mann-Whitney p= 0,9). Conclui-se que a PDT n??o interfere na quimiotaxia de macr??fagos e linf??citos T ativados no tecido periodontal ap??s 7 dias.
Databáze: OpenAIRE
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