Evidence-Based Complementary and Alternative Medicine
| Autor: | Reis, Milena Ramos, Oliveira Filho, Abrahão Alves de, Rodrigues, Lilia Simone Urzedo, Araújo, Jaíse Paiva, Maciel, Priscilla Maria Pereira, Albuquerque, Jamile Morais de, Cehinel Filho, Valdir, Cáceres, Armando, Fregoneze, Josmara Bartolomei, Medeiros, Isac Almeida de, Silva, Darizy Flávia |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Zdroj: | Repositório Institucional da UFBA Universidade Federal da Bahia (UFBA) instacron:UFBA |
| DOI: | 10.1155/2013/147670 |
| Popis: | p. 1-10 Submitted by Suelen Reis (suziy.ellen@gmail.com) on 2014-04-29T17:12:35Z No. of bitstreams: 1 10.11552013-147670.pdf: 1903108 bytes, checksum: 788091f71909c287d22cfd534ac4cbed (MD5) Approved for entry into archive by Patricia Barroso (pbarroso@ufba.br) on 2014-05-01T13:04:25Z (GMT) No. of bitstreams: 1 10.11552013-147670.pdf: 1903108 bytes, checksum: 788091f71909c287d22cfd534ac4cbed (MD5) Made available in DSpace on 2014-05-01T13:04:25Z (GMT). No. of bitstreams: 1 10.11552013-147670.pdf: 1903108 bytes, checksum: 788091f71909c287d22cfd534ac4cbed (MD5) Previous issue date: 2013 Assays in vitro and in vivo were performed on extract from roots and leaves from the Valeriana prionophylla Standl. (VPR and VPF, resp.). In phenylephrine (1 μM) precontracted rings, VPR (0.01–300 μg/mL) induced a concentration-dependent relaxation (maximum response (MR) = 75.4 ± 4.0%, EC50 = 5.97 (3.8–9.3) μg/mL, ]); this effect was significantly modified after removal of the endothelium (EC50 = 39.6 (27.2–57.6) μg/mL, ). However, VPF-induced vasorelaxation was less effective compared to VPR. When rings were preincubated with L-NAME (100 μM) or indomethacin (10 μM), the endothelium-dependent relaxation induced by VPR was significantly attenuated (MR = 20.9 ± 2.3%, 34.2 ± 2.9%, resp., ). In rings denuded endothelium, precontracted with KCl (80 mM), or in preparations pretreated with KCl (20 mM) or tetraethylammonium (1 or 3 mM), the vasorelaxant activity of VPR was significantly attenuated (MR = 40.0 ± 8.2, ; 50.5 ± 6.0%; 49.3 ± 6.4%; 46.8 ± 6.2%; resp., ). In contrast, neither glibenclamide (10 μM), barium chloride (30 μM), nor 4-aminopyridine (1 mM) affected VPR-induced relaxation. Taken together, these results demonstrate that hypotension induced by VPR seems to involve, at least in part, a vascular component. Furthermore, endothelium-independent relaxation induced by VPR involves K+ channels activation, most likely due to BKCa channels, in the rat superior mesenteric artery. |
| Databáze: | OpenAIRE |
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