Molecular detection of Hepatozoon spp. in domestic dogs and wild mammals in southern Pantanal, Brazil with implications in the transmission route
| Autor: | SOUSA, K. C. M. de, FERNANDES, M. P., HERRERA. H. M., BENEVENUTE, J. L., SANTOS, F. M., BARRETO, W. T. G., MACEDOB, G. C., CAMPOSB, J. B., MARTINS, T. F., ROCHA, F. L., PINTOC, C. E. de A., BATTESTI, D. B., PIRANDAF, E. M., CANCADO, P. H. D., MACHADO, R. Z., ANDRÉA, M. R. |
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| Přispěvatelé: | Keyla Carstens Marques de Sousa, Faculdade de Ciências Agrárias e Veterinárias/Universidade Estadual Paulista/UNESP, Marina Pugnaghi Fernandes, Faculdade de Ciências Agrárias e Veterinárias/Universidade Estadual Paulista/UNESP, Heitor Miraglia Herrera, Universidade Católica Dom Bosco, Jyan Lucas Benevenute, Faculdade de Ciências Agrárias e Veterinárias/Universidade Estadual Paulista/UNESP, Filipe Martins Santos, Universidade Católica Dom Bosco, Wanessa Teixeira Gomes Barreto, Universidade Católica Dom Bosco, Gabriel Carvalho Macedob, Universidade Católica Dom Bosco, João Bosco Camposb, Universidade Católica Dom Bosco, Thiago Fernandes Martins, Faculdade de Medicina Veterinária e Zootecnia/Universidade de São Paulo, Fabiana Lopes Rocha, Laboratório de Ecologia Animal/Universidade Federal da Paraíba, Pedro Cordeiro Estrela de Andrade Pintoc, Laboratório de Ecologia Animal/Universidade Federal da Paraíba, Darci Barros Battesti, Laboratório de Parasitologia/Instituto Butantan, Eliane Mattos Pirandaf, Universidade Federal de Mato Grosso do Sul, PAULO HENRIQUE DUARTE CANCADO, CNPGC, Rosangela Zacarias Machado, Faculdade de Ciências Agrárias e Veterinárias/Universidade Estadual Paulista/UNESP, Marcos Rogério Andréa, Faculdade de Ciências Agrárias e Veterinárias/Universidade Estadual Paulista/UNESP. |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: | |
| Zdroj: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA-Alice) Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
| Popis: | Hepatozoon parasites comprise intracellular apicomplexan parasites transmitted to vertebrate animals by ingestion of arthropods definitive hosts. The present work aimed to investigate the occurrence of Hepatozoon spp. in wild animals, domestic dogs and their respective ectoparasites, in southern Pantanal region, central-western Brazil, by molecular techniques. Between August 2013 and March 2015, 31 coatis (Nasua nasua), 78 crab-eating foxes (Cerdocyon thous), seven ocelots (Leopardus pardalis), 42 dogs (Canis lupus familiaris), 110 wild rodents (77 Thichomys fosteri, 25 Oecomys mamorae, and 8 Clyomys laticeps), 30 marsupials (14 Thylamys macrurus, 11 Gracilinanus agilis, 4 Monodelphis domestica and 1 Didelphis albiventris), and 1582 ticks and 80 fleas collected from the sampled animals were investigated. DNA samples were submitted to PCR assays for Hepatozoon spp. targeting 18S rRNA gene. Purified amplicons were directly sequenced and submitted to phylogenetic analysis. A high prevalence of Hepatozoon among carnivores (C. thous [91.02%], dogs [45.23%], N. nasua [41.9%] and L. pardalis [71.4%]) was found. However, ticks and fleas were negative to Hepatozoon PCR assays. By phylogenetic analysis based on 18S rRNA sequences, Hepatozoon sequences amplified from crab-eating foxes, dogs, coatis and ocelots clustered with sequences of H. canis, H. americanum and H. felis. The closely related positioning of Hepatozoon sequences amplified from wild rodents and T. macrurus marsupial to Hepatozoon from reptiles and amphibians suggest a possible transmission of those Hepatozoon species between hosts by ectoparasites or by predation. Hepatozoon haplotypes found circulating in wild rodents seem to present a higher degree of polymorphism when compared to those found in other groups of animals. Although rodents seem not to participate as source of Hepatozoon infection to wild carnivores and domestic dogs, they may play an important role in the transmission of Hepatozoon to reptiles and amphibians in Pantanal biome. Made available in DSpace on 2018-02-06T23:41:06Z (GMT). No. of bitstreams: 1 MoleculardetectionofHepatozoonspp.indomesticdogsandwild.pdf: 1982451 bytes, checksum: 8c94a1fa32b0477e017458b0c0278398 (MD5) Previous issue date: 2018-02-06 |
| Databáze: | OpenAIRE |
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