Mechanisms underlying the antinociceptive, antiedematogenic, and anti-inflammatory activity of the main flavonoid from Kalanchoe pinnata flowers (Lam.) Pers. (fortune sheet)
| Autor: | Ferreira, Raquel Teixeira |
|---|---|
| Přispěvatelé: | Vanderlinde, Frederico Argollo, Silva, Alba Cen?lia Matos da, Malvar, David do Carmo, Pinto, Marcelo Alves, C?rtes, Wellington da Silva |
| Jazyk: | portugalština |
| Rok vydání: | 2016 |
| Předmět: | |
| Zdroj: | Biblioteca Digital de Teses e Dissertações da UFRRJ Universidade Federal Rural do Rio de Janeiro (UFRRJ) instacron:UFRRJ |
| Popis: | Submitted by Celso Magalhaes (celsomagalhaes@ufrrj.br) on 2017-06-21T11:22:42Z No. of bitstreams: 1 2016 - Raquel Teixeira Ferreira.pdf: 4123117 bytes, checksum: d2271c63e8c851f1e25afd9a0bd8cf91 (MD5) Made available in DSpace on 2017-06-21T11:22:42Z (GMT). No. of bitstreams: 1 2016 - Raquel Teixeira Ferreira.pdf: 4123117 bytes, checksum: d2271c63e8c851f1e25afd9a0bd8cf91 (MD5) Previous issue date: 2016-03-30 CAPES - Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior Kalanchoe pinnata (KP) is popularly used for treating inflammatory diseases. This study investigated the antinociceptive and anti-inflammatory potential of the KP flower aqueous extract (KPFE), the ethyl acetate (EtOAcF), butanol (BuOHF) and aqueous (AqF) fractions and the main flavonoid [quercetin 3-O-?-L-arabinopyranosyl (1??) ?-L-rhamnopyranoside] (KPFV) of KP, as well as possible mechanisms of action. Swiss albino mice, male (25-35g) were pretreated with KPFE (30-300 mg/kg, s.c.) producing dose-related inhibition of acetic acid-induced writhing (ID50 164.8 mg/kg), and the subcutaneous administration of KPFE (300 mg/kg), EtOAcF (12 mg/kg), BuOHF (15 mg/kg) or AqF (210 mg/kg) reduced leukocyte migration on carrageenan-induced pleurisy in mice at 56.1%, 47.3%, 39.6% and 43.1%, respectively. At doses of up to 240 times smaller than KPFE, KPFV (0.3-3.0 mg/kg, s.c.), also inhibited leukocyte migration (ID50 1.78 mg/kg). In croton oil-induced ear edema in mice, KPFE (3-30 mg/kg, s.c.) and KPFV (0.3-3.0 mg/kg, s.c.) presented dose-related antiedematogenic activity (KPFE - ID50 4.3 mg/kg, KPFV - ID50 0.76 mg/kg). Treatment with KPFE reduced the MPO and TNF-? concentration in the pleural exudates on carrageenaninduced pleurisy test, while KPFV inhibited both isoforms of cyclooxygenase (COX), with IC50 3.8 x 10-5 M (22.1 ?g/mL) for COX-1 and a maximum COX-2 inhibition of 43.5% (IC50 >8.4 x10-5M or 50 ?g/mL). The selectivity index (COX-1 IC50/COX-2 IC50) was 8,4 x10-5M ou 50 ?g/mL). O ?ndice de seletividade (COX-1 CI50/COX-2 CI50) foi < 0,44. Estes resultados indicam que EAFL e KPFV produziram atividade anti-inflamat?ria e antinociceptiva envolvendo a inibi??o da COX e a redu??o de TNF-?. Este estudo revela que o principal flavonoide presente nas infloresc?ncias e folhas de KP possui papel importante no uso etnomedicinal desta esp?cie nos processos de natureza inflamat?ria |
| Databáze: | OpenAIRE |
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