Antibacterial properties of chitosan-based coatings are affected by spacer-length and molecular weight

Autor: Vaz, Juliana Miguel, 1979, Taketa, Thiago Bezerra, 1988, Hernández Montelongo, Jesús Jacobo, 1979, Cotta, Mônica Alonso, 1963, Beppu, Marisa Masumi, 1972
Přispěvatelé: UNIVERSIDADE ESTADUAL DE CAMPINAS
Rok vydání: 2018
Předmět:
Zdroj: Repositório da Produção Científica e Intelectual da Unicamp
Universidade Estadual de Campinas (UNICAMP)
instacron:UNICAMP
Popis: Agradecimentos: This work was supported by the National Council for Scientific and Technological Development (CNPq) - Brazil (grant #479486/2012-3), Sao Paulo Research Foundation (FAPESP) - Brazil (grants #2010/51748-7 and #2013/10957-0), the Natural Sciences and Engineering Research Council (NSERC) and the Research Center of CHU de Quebec. Thiago Taketa would like to thank Sao Paulo Research Foundation (FAPESP) - Brazil, for grant #2013/05135-1 and CAPES - Brazil (Procad 88882.151600/2017-01). Jacobo Herandez-Montelongo would like to thank Sao Paulo Research Foundation (FAPESP) - Brazil for grant #2013/14888-3. Juliana M. Vaz would like to thank National Council for Scientific and Technological Development (CNPq) - Brazil grant number #249963/2013-2 Abstract: Chitosan is a biopolymer with antibacterial properties, which are dependent on its molecular weight (Mw) and its degree of deacetylation (DDA). When grafted on surfaces as a coating, chitosan antibacterial efficiency is also dependent on the polymer chain conformation on the surface, as the amine groups, responsible of the antibacterial effect, should be available for contact with bacteria. To investigate this behavior, chitosans with different Mw were grafted onto plasma aminated surfaces through three different spacers: glutaric anhydride (GA), poly(ethylene-glycol) bis(carboxymethyl) ether (PEGb), and poly (ethylene-alt-maleic anhydride) (PA). The grafting efficiency was evaluated by X-ray Photoelectron Spectroscopy (XPS), contact angle and Rose Bengal test, while morphological features were assessed by profilometry analyses. Results evidenced a clear influence of the anchor arm length and of the Mw of chitosan both on the grafting efficiency and on the antibacterial behavior. PA CHIMW surface exhibited a better antibacterial response compared to GA and PEGb, which could be correlated to a denser coating coverage as seen by XPS and profilometry results. Further, PA CHIMW coating displayed a higher amine density, thus promoting the interaction with the bacteria cell wall. Based on these results, chitosan-based coatings can then be extended to a wide range of antibacterial applications CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQ FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPES Fechado
Databáze: OpenAIRE
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