Efecto del Medio Hipoosmótico sobre la Vitalidad Espermática en Semen Canino

Autor:	Sánchez R., Alfonso, Zamora D., Patricia
Jazyk:	Spanish; Castilian
Rok vydání:	2016
Předmět:	canine spermatozoa prueba hipoosmótica vitalidad espermática espermatozoide canino hypoosmotic test sperm viability
Zdroj:	Revista de Investigaciones Veterinarias del Perú; Vol. 27 No. 2 (2016); 288-293 Revista de Investigaciones Veterinarias del Perú; Vol. 27 Núm. 2 (2016); 288-293 Revistas Universidad Nacional Mayor de San Marcos Universidad Nacional Mayor de San Marcos instacron:UNMSM
ISSN:	1682-3419 1609-9117
Popis:	The hypoosmotic test is widely used for determining sperm quality in several animal species. The present study aimed to compare the effect of incubation of canine sperm into two hypoosmotic solutions (0 mOsm/l - HOST-s and 150 mOsm/l - HOST) on sperm viability. Fifteen ejaculates (2nd fraction) were obtained by digital manipulation from 10dogs and the spermiograms were considered as normals. A 5 μl aliquots per ejaculate were diluted in 45 μl of each of the two hypoosmotic solutions and incubated at 37 °C for 5 and 45 min respectively. Sperm viability was assessed using eosin-nigrosin staining. Results of the hypoosmotic test were 92.1 and 90.1% of dilated spermatozoa for HOST-s and HOST respectively (p
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=od______3056::ab0b6b15abd753de664a6b8d8c015dbe https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/11649 Zobrazit plný text záznamu