Toxicological effects of bisulfite and other toxic agents on the cytoplasmic glycerol-3-phosphate dehydrogenase of baker's yeast
| Autor: | Gattas, Edwil Aparecida de Lucca [UNESP], Peres, M. F S [UNESP] |
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| Přispěvatelé: | Universidade Estadual Paulista (Unesp) |
| Jazyk: | portugalština |
| Rok vydání: | 2006 |
| Předmět: |
culture medium
concentration (parameters) Inhibitors glycerol fungus culture Saccharomyces cerevisiae toxicity testing glycerol 3 phosphate dehydrogenase carbon source enzyme synthesis bisulfite glucose toxic substance citric acid cycle Glycerol-3-phosphate dehydrogenase fermentation Baker's yeast acetaldehyde |
| Zdroj: | Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| Popis: | Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-27T11:22:05Z No. of bitstreams: 0Bitstream added on 2014-05-27T14:45:48Z : No. of bitstreams: 1 2-s2.0-35348993198.pdf: 77237 bytes, checksum: 2f0695884100b959e1c7c8a1d89a151d (MD5) Made available in DSpace on 2014-05-27T11:22:05Z (GMT). No. of bitstreams: 0 Previous issue date: 2006-12-01 The synthesis of intracellular glycerol-3-phosphate dehydrogenase (EC 1.1.1.8) in baker's yeast was investigated in submerged culture supplied with glucose or glycerol as sole carbon sources. Inhibitors of the glycolytic pathway, Krebs cycle and respiratory chain did not stimulate glycerol-3-phosphate dehydrogenase synthesis when added in low concentrations in up 7.5 × 10 -5 mol/L. The repression exercised by glucose on the synthesis of glycerol-3-phosphate dehydrogenase in YP-glucose medium was reduced by the addition of fermentation products and of sodium bisulfite. Synthesis of the enzyme was raised 22-110%. However, in YP-glycerol medium, the addition of 0.06% (w/v) sodium bisulfite reduced (29%) the synthesis of the enzyme, while 0.012% (v/v) acetaldehyde stimulated the synthesis of glycerol-3-phosphate dehydrogenase by 12%. Departamento de Alimentos e Nutrição Faculdade de Ciências Farmacêuticas Universidade Estadual Paulista, UNESP, Araraquara, SP Departamento de Alimentos e Nutrição Faculdade de Ciências Farmacêuticas Universidade Estadual Paulista, UNESP, Rodovia Araraquara-Jaú, km 1, CEP 14801-902 - Araraquara - SP Departamento de Alimentos e Nutrição Faculdade de Ciências Farmacêuticas Universidade Estadual Paulista, UNESP, Araraquara, SP Departamento de Alimentos e Nutrição Faculdade de Ciências Farmacêuticas Universidade Estadual Paulista, UNESP, Rodovia Araraquara-Jaú, km 1, CEP 14801-902 - Araraquara - SP |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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