Transmissibility of Small Ruminants Lentivirus in kids by experimentally infected semen

Autor: Hasegawa, Marjorie Y., Lara, Maria do C. C. S. H., Gaeta, Natalia C., Marques, Julia A., Ribeiro, Bruno L. M., Rossi, Rodolfo S. [UNESP], Marques, Eduardo C., Gregory, Lilian
Přispěvatelé: Universidade de São Paulo (USP), Inst Biol, Universidade Estadual Paulista (Unesp)
Jazyk: portugalština
Rok vydání: 2017
Předmět:
Zdroj: Web of Science
Repositório Institucional da UNESP
Universidade Estadual Paulista (UNESP)
instacron:UNESP
ISSN: 0100-736X
Popis: Made available in DSpace on 2018-11-26T17:42:25Z (GMT). No. of bitstreams: 0 Previous issue date: 2017-08-01. Added 1 bitstream(s) on 2021-07-15T14:32:08Z : No. of bitstreams: 1 S0100-736X2017000800805.pdf: 863913 bytes, checksum: e0876583dbecff3fd8640f585619365a (MD5) Caprine Arthritis Encephalitis is a multisystemic infectious disease, caused by a lentivirus. The objective of this study was to evaluate the transmissibility of caprine lentivirus to goats and their offspring, through experimentally infected semen. Therefore, eleven free- CAEV goats were artificially inseminated using semen from a free-CAEV buck experimentally infected with CAEV-Cork strain (experimental group one). Pregnancy was confirmed in only six goats and their offspring (n= 6) constituted the experimental group two. Two free-CAEV females were artificially inseminated with semen from the same seronegative buck, without viral inoculum to constitute the control group. The diagnosis of caprine lentivirus infection was performed using AGID, cELISA and nested-PCR. All females were monitored for 210 days after artificial insemination. Kids were immediately separated from their mothers after birth, and monitored at zero time, 15 days old and monthly until 12 months old. Regarding goat samples, 56.96% (9/159) were positive in cELISA, 24.05% (38/158) were positive in IDGA and none was positive in nested-PCR. Regarding to the offspring samples, 11.28% (15/133) and 5.26% (7/133) were positive in nested-PCR and IDGA, respectively, while no sample was positive in cELISA. The control group showed no positives in the three techniques. The positivity observed to nested-PCR may show its importance to identify infected, but seronegative animals, in late seroconversion situations. According to results, the transmission of caprine lentivirus to offspring and their mothers through infected semen is possible. Univ Sao Paulo, Dept Clin Med, Fac Med Vet & Zootecnia, Ave Prof Dr Orlando Marques Paiva 87,Cidade Univ, BR-05508270 Sao Paulo, SP, Brazil Inst Biol, Lab Raiva & Encefalites, Ave Conselheiro Rodrigues Alves 1-252, BR-04014002 Sao Paulo, SP, Brazil Univ Estadual Paulista, Dept Higiene Vet & Saude Publ, Fac Med Vet & Zootecnia Botucatu, Julio de Mesquita Filho S-N, BR-18618970 Botucatu, SP, Brazil Univ Estadual Paulista, Dept Higiene Vet & Saude Publ, Fac Med Vet & Zootecnia Botucatu, Julio de Mesquita Filho S-N, BR-18618970 Botucatu, SP, Brazil
Databáze: OpenAIRE
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