Evaluation of Physiological Parameters and Muscle?s Mitochondrial Metabolism of Eventing Horses Submitted to Intense Exercise

Autor: Miranda, Ana Claudia Tavares
Přispěvatelé: Almeida, Fernando Queiroz de, Ara?jo, Alexandre Herculano Borges de, Galina Filho, Ant?nio, Baldani, Cristiane Divan
Jazyk: portugalština
Rok vydání: 2014
Předmět:
Zdroj: Biblioteca Digital de Teses e Dissertações da UFRRJ
Universidade Federal Rural do Rio de Janeiro (UFRRJ)
instacron:UFRRJ
Popis: Submitted by Celso Magalhaes (celsomagalhaes@ufrrj.br) on 2019-08-12T17:23:44Z No. of bitstreams: 1 2014 - Ana Cl?udia Tavares Miranda.pdf: 2093398 bytes, checksum: 3b18f0581b35fa8a7a1a303165cd88fb (MD5) Made available in DSpace on 2019-08-12T17:23:44Z (GMT). No. of bitstreams: 1 2014 - Ana Cl?udia Tavares Miranda.pdf: 2093398 bytes, checksum: 3b18f0581b35fa8a7a1a303165cd88fb (MD5) Previous issue date: 2014-02-17 Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES The study was carried out to evaluate the changes in physiological parameters muscle?s mitochondrial metabolism related to physical performance before and after maximal exercise treadmill test in eventing horses. The research was conducted at Laboratory of Horse Performance Evaluation, Brazilian Army Cavalry School, RJ, Brazil. Fourteen Brazilian Sport Horses were used, age ranging from 4 to 6 years old, and beginners at any exercise program. The incremental test consisted of three stages: warm up ? walk at 1.7m/s for 2 minutes, trot at 4 m/s for 2 minutes and, with the treadmill inclined at 3%, trot at 4 m/s for 2 minutes; gallops ? maintaining the inclination, started at a 5m/s speed, increasing the velocity 1m/s every minute until 8m/s, where the horses remained until the exhaustion; and recovery ? walk at 1.7m/s for 10 minutes. The samples were collected before and immediately after the end of the test. Blood analyzes were measured in a spectrophotometer using commercial kit. Heart rate was monitored with frequency counter. Muscle samples were obtained from the middle gluteal muscle, it was transferred into ice-cold maintenance solution BIOPS. Mitochondrial respiration was assessed with O2K oxygraph of high resolution (Oroboros). The protocol was: Piruvate/Malate (5/5mM), Adenosine diphosphate (1mM), Succinate (20mM), Adenosine diphosphate (1mM), Cytochrome c (0,01mM), Oligomycin (2?g/ml), Carbonyl-cyane-phenyl-hydrozone (0,001mM), Rotenone (0,5?M), Antimycin A (2,5?M); in MIR05. The results were normalized per segundo per gram of wet weight and expressed as mean and SD. Paired t?test was used and p0,05). Os valores de pH e HCO3- diminuiram (p
Databáze: OpenAIRE
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