Estudo qu??mico e atividades farmacol??gicas em esponjas de ??gua doce da Amaz??nia

Autor: Bolson, Glaucia Cristina Man??o da Costa, https://orcid.org/0000-0003-1732-4346
Přispěvatelé: Veiga Junior, Valdir Flor??ncio, Lima, Emerson Silva, Guimar??es, Anderson Cavalcante, Nunomura, Rita de C??ssia Saraiva, Canhoto, Olinda Maria Figueira
Jazyk: portugalština
Rok vydání: 2020
Předmět:
Zdroj: Biblioteca Digital de Teses e Dissertações da UFAM
Universidade Federal do Amazonas (UFAM)
instacron:UFAM
Popis: Submitted by Glaucia Bolson (glaucia.manco@gmail.com) on 2021-07-07T18:52:23Z No. of bitstreams: 3 Tese_Glaucia Bolson.pdf: 67619659 bytes, checksum: 163cf7179dbd75defd443d99a30c467d (MD5) Glaucia Bolson-Carta de encaminhamento.pdf: 41906 bytes, checksum: 790947589a8e4bbe4600c807f24a3d97 (MD5) Glaucia Bolson-Folha de aprova????o assinada.pdf: 458386 bytes, checksum: 271294bce649af96d81a21c535bfa143 (MD5) Approved for entry into archive by PPGIF Inova????o Farmac??utica (ppgif@ufam.edu.br) on 2022-03-25T13:08:20Z (GMT) No. of bitstreams: 3 Tese_Glaucia Bolson.pdf: 67619659 bytes, checksum: 163cf7179dbd75defd443d99a30c467d (MD5) Glaucia Bolson-Carta de encaminhamento.pdf: 41906 bytes, checksum: 790947589a8e4bbe4600c807f24a3d97 (MD5) Glaucia Bolson-Folha de aprova????o assinada.pdf: 458386 bytes, checksum: 271294bce649af96d81a21c535bfa143 (MD5) Approved for entry into archive by Divis??o de Documenta????o/BC Biblioteca Central (ddbc@ufam.edu.br) on 2022-03-25T19:02:01Z (GMT) No. of bitstreams: 3 Tese_Glaucia Bolson.pdf: 67619659 bytes, checksum: 163cf7179dbd75defd443d99a30c467d (MD5) Glaucia Bolson-Carta de encaminhamento.pdf: 41906 bytes, checksum: 790947589a8e4bbe4600c807f24a3d97 (MD5) Glaucia Bolson-Folha de aprova????o assinada.pdf: 458386 bytes, checksum: 271294bce649af96d81a21c535bfa143 (MD5) Made available in DSpace on 2022-03-25T19:02:01Z (GMT). No. of bitstreams: 3 Tese_Glaucia Bolson.pdf: 67619659 bytes, checksum: 163cf7179dbd75defd443d99a30c467d (MD5) Glaucia Bolson-Carta de encaminhamento.pdf: 41906 bytes, checksum: 790947589a8e4bbe4600c807f24a3d97 (MD5) Glaucia Bolson-Folha de aprova????o assinada.pdf: 458386 bytes, checksum: 271294bce649af96d81a21c535bfa143 (MD5) Previous issue date: 2020-02-27 FAPEAM - Funda????o de Amparo ?? Pesquisa do Estado do Amazonas CNPQ - Conselho Nacional de Desenvolvimento Cient??fico e Tecnol??gico Sponges (phylum Porifera), are aquatic animals, with marine and freshwater specimens. Although found in several regions, freshwater sponges are one of the least known faunas in the world. Most studies are reported with marine sponges. Sponges have an enormous biotechnological potential in the search for natural products due to the various biological activities. Just like marine sponges, which feature various bioactive compounds (eg, alkaloids, macrolides, peptides, polyketides, quinines, sterols and terpenoids), species of freshwater sponges are reported to be chemically made up of fatty acids and sterols. In the chemical study of freshwater sponges, sterols deserve attention due to the great diversity they present and the great biological potential. The compounds produced by sponges are important natural resources, which can lead to the production of drugs to help mainly in the treatment of neurodegenerative diseases and malaria. Due to the absence of chemical and biological studies of these organisms in the Amazon, it was the reason for carrying out work in the region due to their great occurrence. The present work aimed to characterize and identify chemical compounds from the metabolism of sponges by means of metabolomic analysis and to evaluate the inhibition of acetylcholinesterase and butyrylcholinesterase (AChE and BuChE) and the inhibition of Plasmodium falciparum, of methanol extracts from sponges of freshwater from the Amazon (Drulia uruguayensis, Drulia cristata, Drulia browni and Metania reticulata). To verify the chemical profile of the extracts, analyzes were performed in mass spectrometry by direct insertion and high- performance liquid chromatography coupled to a high-resolution mass spectrometry detector. To verify the inhibitory activity of acetylcholinesterase, the Ellman method was used, modified by Lima, and the inhibitory activity of P. falciparum was used according to the FIOCRUZ-AM platform. All extracts inhibited AChE, however, no activity was observed in relation to BChE. The extract of Drulia uruguayensis showed greater inhibition of AChE, with IC50 = 1.04 mg/mL. For antiplasmodial activity, all species showed inhibition of P. falciparum, but the sample of Metania reticulata was the most efficient with IC50 = 2.7 ??g/mL. The metabolomic analysis carried out by mass spectrometry showed the presence of biomarkers that helped differentiate species, the biomarkers found in the active extracts were identified as fatty acids and sterols. The metabolites of freshwater sponges have few studies in the literature, however with the results obtained, it has helped in the enrichment and significance of studies with freshwater sponges, which already prove that in the Amazon region sponges have metabolites with similar activities to marine sponge. As esponjas (filo Por??fera), s??o animais aqu??ticos, com exemplares marinhos e de ??gua doce. Embora encontradas em v??rias regi??es, as esponjas de ??guas doce constituem uma das faunas menos conhecidas em todo o mundo. Sendo a maioria dos estudos relatados com esponjas marinhas. As esponjas possuem um enorme potencial biotecnol??gico na pesquisa de produtos naturais devido ??s diversas atividades biol??gicas. Assim como as esponjas marinhas, que apresentam v??rios compostos bioativos (por exemplo, alcal??ides, macr??lidos, pept??deos, policet??deos, quininas, ester??is e terpen??ides), esp??cies de esponjas de ??gua doce s??o relatadas como quimicamente constitu??das por ??cidos graxos e ester??is. No estudo qu??mico de esponjas de ??gua doce, os ester??is merecem aten????o devido ?? grande diversidade que apresentam e o grande potencial biol??gico. Os compostos produzidos por esponjas s??o recursos naturais importantes, podendo levar ?? produ????o de medicamentos para auxiliar principalmente no tratamento de doen??as neurodegenerativas e mal??ria. Devido a aus??ncia de estudos qu??micos e biol??gicos desses organismos na Amaz??nia, foi que motivou a realiza????o de trabalhos na regi??o devido a grande ocorr??ncia destes. O presente trabalho teve como objetivo, caracterizar e identificar composto qu??micos a partir do metabolismo de esponjas por meio de an??lise metabol??mica e avaliar a inibi????o da acetilcolinesterase e butirilcolinesterase (AChE e BuChE) e a inibi????o do Plasmodium falciparum, dos extratos em metanol de esponjas de ??gua doce da Amaz??nia (Drulia uruguayensis, Drulia cristata, Drulia browni e Metania reticulata). Para verificar o perfil qu??mico dos extratos, foram realizadas an??lises em espectrometria de massas por inser????o direta e cromatografia l??quida de alta efici??ncia acoplado a um detector de espectrometria de massas de alta resolu????o. Para verificar ?? atividade inibit??ria da acetilcolinesterase foi utilizado o m??todo de Ellman, modificado por Lima e a atividade inibit??ria do P. falciparum foi de acordo com a plataforma da FIOCRUZ-AM. Todos os extratos inibiram a AChE, por??m, nenhuma atividade foi observada em rela????o ?? BChE. O extrato de Drulia uruguayensis apresentou maior inibi????o a AChE, com IC50 = 1,04 mg/mL. Para atividade antiplasm??dica, todas as esp??cies apresentaram inibi????o a P. falciparum, mas a amostra de Metania reticulata foi a mais eficiente com IC50 = 2,7 ??g/mL. A an??lise metabol??mica realizada por espectrometria de massa evidenciou a presen??a de biomarcadores que auxiliaram na diferencia????o das esp??cies, os biomarcadores encontrados nos extratos ativos foram identificados como ??cidos graxos e ester??is. Os metab??litos das esponjas de ??gua doce apresentam poucos estudos na literatura, no entanto com os resultados obtidos, auxiliou no enriquecimento e signific??ncia dos estudos com esponjas de ??gua doce, que j?? comprovam que na regi??o Amaz??nica as esponjas possuem metab??litos com atividades semelhantes ??s marinhas.
Databáze: OpenAIRE
Externí odkaz: