Journal of Medical Microbiology
| Autor: | Pacheco, Luis Gustavo Carvalho, Pena, Roberta R., Castro, Thiago Luiz de Paula, Dorella, Fernanda Alves, Bahia, Robson Cerqueira, Carminati, Renato, Nascimento, Roberto José Meyer |
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| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: | |
| Zdroj: | Repositório Institucional da UFBA Universidade Federal da Bahia (UFBA) instacron:UFBA |
| DOI: | 10.1099/jmm.0.46997-0 |
| Popis: | Texto completo: acesso restrito. p. 480-486 Submitted by Suelen Reis (suziy.ellen@gmail.com) on 2014-01-29T14:51:20Z No. of bitstreams: 1 10.1099-jmm.0.46997-0.pdf: 192545 bytes, checksum: b902261a9419061456f5b6bb3ce2aa91 (MD5) Approved for entry into archive by Alda Lima da Silva (sivalda@ufba.br) on 2014-02-13T17:49:33Z (GMT) No. of bitstreams: 1 10.1099-jmm.0.46997-0.pdf: 192545 bytes, checksum: b902261a9419061456f5b6bb3ce2aa91 (MD5) Made available in DSpace on 2014-02-13T17:49:33Z (GMT). No. of bitstreams: 1 10.1099-jmm.0.46997-0.pdf: 192545 bytes, checksum: b902261a9419061456f5b6bb3ce2aa91 (MD5) Previous issue date: 2007 Corynebacterium pseudotuberculosis is the aetiological agent of caseous lymphadenitis (CLA), a debilitating disease of sheep and goats. Accurate diagnosis of CLA primarily relies on microbiological examination, followed by biochemical identification of isolates. In an effort to facilitate C. pseudotuberculosis detection, a multiplex PCR (mPCR) assay was developed targeting three genes of this bacterium: the 16S rRNA gene, rpoB and pld. This method allowed efficient identification of 40 isolates of this bacterium that had been identified previously by biochemical testing. Analysis of taxonomically related species did not generate the C. pseudotuberculosis mPCR amplification profile, thereby demonstrating the assay's specificity. As little as 1 pg of C. pseudotuberculosis genomic DNA was detected by this mPCR assay, demonstrating the sensitivity of the method. The detection limit in clinical samples was estimated to be 103 c.f.u. C. pseudotuberculosis could be detected directly in pus samples from infected sheep and goats (n=56) with a high diagnostic sensitivity (94.6 %). The developed assay significantly improves rapid C. pseudotuberculosis detection and could supersede bacteriological culture for microbiological and epidemiological diagnosis of CLA. |
| Databáze: | OpenAIRE |
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