RAPID COMMUNICATION: Multi-breed validation study unraveled genomic regions associated with puberty traits segregating across tropically adapted breeds

Autor: Melo, Thaise P. [UNESP], Fortes, Marina R. S., Fernandes Junior, Gerardo A. [UNESP], Albuquerque, Lucia G. [UNESP], Carvalheiro, Roberto [UNESP]
Přispěvatelé: Universidade Estadual Paulista (Unesp), School of Chemistry and Molecular Biosciences, Queensland Alliance for Agriculture and Food Innovation, National Council for Scientific and Technological Development (CNPq)
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Zdroj: Scopus
Repositório Institucional da UNESP
Universidade Estadual Paulista (UNESP)
instacron:UNESP
Popis: Made available in DSpace on 2019-10-06T16:39:35Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-07-02 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) An efficient strategy to improve QTL detection power is performing across-breed validation studies. Variants segregating across breeds are expected to be in high linkage disequilibrium (LD) with causal mutations affecting economically important traits. The aim of this study was to validate, in a Tropical Composite cattle (TC) population, QTL associations identified for sexual precocity traits in a Nellore and Brahman meta-analysis genome-wide association study. In total, 2,816 TC, 8,001 Nellore, and 2,210 Brahman animals were available for the analysis. For that, genomic regions significantly associated with puberty traits in the meta-analysis study were validated for the following sexual precocity traits in TC: age at first corpus luteum (AGECL), first postpartum anestrus interval (PPAI), and scrotal circumference at 18 months of age (SC). We considered validated QTL those underpinned by significant markers from the Nellore and Brahman meta-analysis (P ≤ 10-4) that were also significant for a TC trait, i.e., presenting a P-value of ≤10-3 for AGECL, PPAI, or SC. We also considered as validated QTL those regions where significant markers in the reference population were at ±250 kb from significant markers in the validation population. Using this criteria, 49 SNP were validated for AGECL, 4 for PPAI, and 14 for SC, from which 5 were in common with AGECL, totaling 62 validated SNP for these traits and 30 candidate genes surrounding them. Considering just candidate genes closest to the top SNP of each chromosome, for AGECL 8 candidate genes were identified: COL8A1, PENK, ENSBTAG00000047425, BPNT1, ADAMTS17, CCHCR1, SUFU, and ENSBTAG00000046374. For PPAI, 3 genes emerged as candidates (PCBP3, KCNK10, and MRPS5), and for SC 8 candidate genes were identified (SNORA70, TRAC, ASS1, BPNT1, LRRK1, PKHD1, PTPRM, and ENSBTAG00000045690). Several candidate regions presented here were previously associated with puberty traits in cattle. The majority of emerging candidate genes are related to biological processes involved in reproductive events, such as maintenance of gestation, and some are known to be expressed in reproductive tissues. Our results suggested that some QTL controlling early puberty seem to be segregating across cattle breeds adapted to tropical conditions. Department of Animal Science School of Agricultural and Veterinarian Sciences FCAV/UNESP Sao Paulo State University University of Queensland School of Chemistry and Molecular Biosciences University of Queensland Queensland Alliance for Agriculture and Food Innovation National Council for Scientific and Technological Development (CNPq) Department of Animal Science School of Agricultural and Veterinarian Sciences FCAV/UNESP Sao Paulo State University
Databáze: OpenAIRE
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