Utiliza??o do peric?rdio de avestruz na aortoplastia em coelhos: avali??o dopplerfluxom?trica e estrutural

Autor: Gomes, Marcelo Salvador
Přispěvatelé: Figueiredo, Marcelo Abidu, Gitrana, Lycia de Brito, Santos, Clarice Machado dos, Palhano, Helcimar Barbosa, Rodrigues, Mauro Roberto, Alonso, Luciano da Silva
Jazyk: portugalština
Rok vydání: 2017
Předmět:
Zdroj: Biblioteca Digital de Teses e Dissertações da UFRRJ
Universidade Federal Rural do Rio de Janeiro (UFRRJ)
instacron:UFRRJ
Popis: Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2021-04-18T23:48:19Z No. of bitstreams: 1 2017 - Marcelo Salvador Gomes.pdf: 2123617 bytes, checksum: 57aadc53a31b74b18e5a68bc4a7b2bc7 (MD5) Made available in DSpace on 2021-04-18T23:48:21Z (GMT). No. of bitstreams: 1 2017 - Marcelo Salvador Gomes.pdf: 2123617 bytes, checksum: 57aadc53a31b74b18e5a68bc4a7b2bc7 (MD5) Previous issue date: 2017-10-06 Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior, CAPES, Brasil. The use of biological membranes in vascular surgery has been used on a large scale in veterinary and human medicine. The objectives of this study were to evaluate the hemodynamic, morphometric and ultrastructural patterns of the abdominal aorta region of rabbits, where the pericardium of ostrich was implanted. The replacement of tissues or organs in animals or in humans is a great challenge to be overcome by the current surgery. This replacement is indicated when loss of anatomical structure occurs. Many materials have been tested and are used for this purpose, whether synthetic or biological. The bovine pericardium is the most researched and employed material in implants and to make prosthesis due to its features: easy acquisition, low cost and by possessing a thick resistant layer of collagen and elastic fibers that intersect at various angles. In this project, fragments of ostrich pericardium, treated and preserved in a 0.5% glutaraldehyde solution will be used and implanted in the abdominal portion of rabbits? aorta. Two groups will be established: Group I (control), group II (ostrich pericardium). The rabbits of Group II will be submitted to standard anesthetic Protocol and celiotomy to aorta where an incision of about 1 cm and aortoplasty using ostrich pericardium fragment with adhesive or surgical suture thread will be made. The animals was also be evaluated by Doppler for characterization of morphometric and arterial hemodynamic parameters. We used a Sonosite Titan, with linear multifrequency transducer.After completing the different healing times (7, 14 and 21 days) the animals from Group I and II was submitted to assisted death through overdose of intravenous thiopental sodium and necropsied to collect fragments of aorta (in Group II, where the implants were made). The fragments was cleaved across and fixed in a 10% buffered formaldehyde solution. All incisions will be processed to be included into paraffin following the histological technique routine, stained by histological and histochemical techniques and. The aorta of animals submitted to the implant with the pericardium of ostrich had a strong inflammatory reaction in the portion facing the vessel lumen on the 7th day, as well as a disorganization of inner and median layers. In addition, the pericardium remained healthy without blood extravasation. On the 14th day, the reorganization of inner and median layer was observed and there were still regions with inflammatory cells. The pericardium has remained healthy. On the 21st day, the aorta had its layers already consolidated. In the abdominal aortic artery was seen a laminar flow profile. The diameter of the aorta varied between 0.31 and 0.54 centimeters. The mean 0.431 ? 0.030 in group I, 0.404 ? 0.100 in group II, 0.408 ? 0.008 in group III, in group IV was 0.431 ? 0.006 (p = 0.2095). The thickness of the aorta varied between 0,10 and 0,29 centimeters The mean and standard deviation were the 0.107 ? 0.011 in group I, 0.105 ? 0.007 in group II, 0.108 ? 0.008 in group III and 0.121 ? 0.006 in group IV (p
Databáze: OpenAIRE
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