Inhame (Dioscorea cayennensis) cultivado na Paraíba: caracterização e produção de concentrado proteico para prospecção de peptídeos bioativos obtidos através de digestão simulada in vitro
| Autor: | Nascimento, Edilza Silva do |
|---|---|
| Přispěvatelé: | Gadelha, Carlos Alberto de Almeida, Pacheco, Maria Teresa Bertoldo |
| Jazyk: | portugalština |
| Rok vydání: | 2020 |
| Předmět: | |
| Zdroj: | Biblioteca Digital de Teses e Dissertações da UFPB Universidade Federal da Paraíba (UFPB) instacron:UFPB |
| Popis: | The yam is an important tuber in Brazil Northeast food culture, also being considered a functional food, as it has proteins with potential bioactive properties. The objective of this study was to characterize the peptide profile and perform the bioprospection of bioactive peptides obtained from in vitro simulated gastrointestinal digestion of yam proteins (Dioscorea cayennensis) grown in Brazil. Therefore, the sample obtained was initially analyzed for partial chemical composition, followed by obtaining the flour for the yam extract and protein concentrate (YPC) preparation, submitted to simulated digestion in vitro, and finally the characterization and profile of the obtained peptides were performed. The protein extract was subjected to isoelectric precipitation to obtain the YPC, which was then dialyzed and freeze-dried. The YPC was submitted to simulated gastrointestinal (GI) digestion, generating two phases: the gastric phase hydrolysate (GPH) and continuous hydrolysis until the gastrointestinal phase hydrolysate (GIPH). The samples: YPC, GPH and GIPH were submitted to analysis of protein and peptide profile: total amino acids and free amino acids (FAA); molecular mass (MM); hydrophobicity by reverse phase chromatography and electrophoresis (Tricine-SDS-PAGE); protein profile (two-dimensional gel electrophoresis) and peptide profile (nano-LC-ESI-MS/MS, MALDI-TOF). The identified peptides were submitted to the bioactivity prediction through the Bioactive Peptides Database (BIOPEP) and PeptideRanker (inhibitory activity of the angiotensin-converting enzyme-ACE). To validate results obtained in silico, analysis were performed to determine biological activities in vitro: antioxidant (DPPH, ABTS, ORAC and protection from DNA damage), antibacterial and ACE’s inhibitor. The results of 2D-electrophoresis show the presence of the dioscorin protein, as the yam’s major protein. The YCP resulted in 64.0 % protein, with all essential amino acids with an appropriate score according to FAO, except sulfur amino acids. After the GI digestion of the YPC, a significant increase in the release (p |
| Databáze: | OpenAIRE |
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