Green production of cladribine by using immobilized 2 0 -deoxyribosyltransferase from lactobacillus delbrueckii stabilized through a double covalent/entrapment technology
| Autor: | Wanda Rivero, Cintia, García, Natalia Soledad, Fernández-Lucas, Jesús, Betancor, Lorena, Romanelli, Gustavo, Trelles, Jorge Abel |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: | |
| Zdroj: | Biomolecules REDICUC-Repositorio CUC Corporación Universidad de la Costa instacron:Corporación Universidad de la Costa |
| Popis: | Nowadays, enzyme-mediated processes offer an eco-friendly and efficient alternative to the traditional multistep and environmentally harmful chemical processes. Herein we report the enzymatic synthesis of cladribine by a novel 2′-deoxyribosyltransferase (NDT)-based combined biocatalyst. To this end, Lactobacillus delbrueckii NDT (LdNDT) was successfully immobilized through a two-step immobilization methodology, including a covalent immobilization onto glutaraldehyde-activated biomimetic silica nanoparticles followed by biocatalyst entrapment in calcium alginate. The resulting immobilized derivative, SiGPEI 25000-LdNDT-Alg, displayed 98% retained activity and was shown to be active and stable in a broad range of pH (5–9) and temperature (30–60 °C), but also displayed an extremely high reusability (up to 2100 reuses without negligible loss of activity) in the enzymatic production of cladribine. Finally, as a proof of concept, SiGPEI 25000-LdNDT-Alg was successfully employed in the green production of cladribine at mg scale. |
| Databáze: | OpenAIRE |
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