Electrophoretic study of the genome of human rotaviruses from Rio de Janeiro, S?o Paulo and Par?, Brazil
| Autor: | Pereira, H. G, Azeredo, Rosete S, Leite, J. P. G, Candeias, J. A. N, R?cz, Maria L, Linhares, Alexandre da Costa, Gabbay, Yvone Benchimol, Trabulsi, J. R |
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| Jazyk: | angličtina |
| Rok vydání: | 1983 |
| Předmět: |
S?o Paulo (SP)
Eletroforese / m?todos Rotav?rus virus diseases Infec??es por Rotav?rus / microbiologia Rio de Janeiro (RJ) humanities Par? (PA) Gastroenterite / microbiologia Rotav?rus / isolamento e purifica??o Rotav?rus / gen?tica parasitic diseases Ensaio de Imunoadsor??o Enzim?tica / m?todos geographic locations Rotav?rus / classifica??o |
| Zdroj: | Repositório Digital do Instituto Evandro Chagas (Patuá) Instituto Evandro Chagas (IEC) instacron:IEC |
| Popis: | Oswaldo Cruz Foundation. Department of Virology. Rio de Janeiro, RJ, Brazil. Oswaldo Cruz Foundation. Department of Virology. Rio de Janeiro, RJ, Brazil. Oswaldo Cruz Foundation. Department of Virology. Rio de Janeiro, RJ, Brazil. University of S?o Paulo. Department of Biomedical Sciences. S?o Paulo, SP, Brazil. University of S?o Paulo. Department of Biomedical Sciences. S?o Paulo, SP, Brazil. Minist?rio da Sa?de. Funda??o Servi?os de Sa?de P?blica. Instituto Evandro Chagas. Bel?m, PA, Brasil. Minist?rio da Sa?de. Funda??o Servi?os de Sa?de P?blica. Instituto Evandro Chagas. Bel?m, PA, Brasil. Escola Paulista de Medicina. Department of Microbiology Immunology and Parasitology. S?o Paulo, SP, Brazil. Human rotaviruses from the states of Rio de Janeiro, S?o Paulo and Par? of Brazil were analysed by RNA electrophoresis. At least some bands characteristic of rotavirus double-stranded RNA were detected in 138 (86.8%) of 159 faecal samples in which the presence of rotavirus had been demonstrated by enzyme immunoassay. Of the RNA-positive samples, 18 (13.0%) were classified as subgroup 1, 94 (68.1%) as subgroup 2, and 26 (18.8%) could not be classified due to absence of visible bands 10 and 11. Subgroup 2 was more frequent in the three states. All strains of subgroup 1 detected in Rio de Janeiro were associated with a single short-lived school outbreak. All strains of subgroup 1 resembled each other in electrophoretic pattern, irrespective of geographical origin, although minor differences could be detected by co-electrophoresis. Subgroup 2, on the other hand, showed a great degree of electrophoretic heterogeneity and could be divided into several sub-categories. |
| Databáze: | OpenAIRE |
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