Resistance of Chrysodeixis includens (Lepidoptera: noctuidae) to insecticides: survey, inheritance and fitness cost

Autor:	Stacke, Regis Felipe
Přispěvatelé:	Guedes, Jerson Carus, Guedes, Raul Narciso, Franco, Cláudio Roberto, Stürmer, Glauber Renato, Arnemann, Jonas André
Jazyk:	portugalština
Rok vydání:	2020
Předmět:	Modulador de canal de sódio Soybean looper Lagarta-falsa-medideira Chemical control Herança da resistência CIENCIAS AGRARIAS::AGRONOMIA [CNPQ] Resistance inheritance Controle químico Sodium channel modulator Manejo da resistência de insetos Insect resistance management
Zdroj:	Biblioteca Digital de Teses e Dissertações do UFSM Universidade Federal de Santa Maria (UFSM) instacron:UFSM
Popis:	Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES Chrysodeixis includens (Walker, 1858) (Lepidoptera: Noctuidae), is an important pest of soybean and cotton in Brazil. The use of insecticides is one of the main control tactics against this pest. To support Integrated Pest Management (IPM) and Insect Resistance Management (IRM) programs, in the first study, we characterized the susceptibility of Brazilian populations of C. includens to insecticides and; in the second study, we selected a strain with resistance to lambda-cyhalothrin and evaluated the inheritance, fitness costs and cross-resistance to other pyrethroids. In the first study, field populations were collected from soybean fields during the 2016–2017 and 2017–2018 crop seasons. In the laboratory, late L2/early L3 larvae were exposed to insecticides in diet-overlay or topical bioassays. Field populations of C. includens showed low levels of resistance to spinetoram (LC50 = 0.074 to 0.25 μg a.i. cm-2 ), indoxacarb (LC50 = 0.46 to 0.94 μg a.i. cm-2 ), thiodicarb (LC50 = 9.14 to 36.61 μg a.i. cm-2 ), chlorantraniliprole (LC50 = 0.15 to 0.57 μg a.i. cm-2 ), flubendiamide (LC50 = 0.45 to 2.01 μg a.i. cm-2 ), and chlorfenapyr (LC50 = 0.15 to 0.25 μg a.i. cm-2 ); the resistance ratios (RR) were less than 16-fold. In contrast, C. includens populations showed high levels of resistance to lambda-cyhalothrin (LC50 = 3.71 to 9.54 μg a.i. cm-2 ), methoxyfenozide (LC50 = 0.67 to 4.23 μg a.i. cm-2 ), novaluron (LC50 = 27.52 to 77.63 μg a.i. cm-2 ), and teflubenzuron (LC50 = 13.41 to 73.02 μg a.i. cm-2 ). The RRs relative to a susceptible population was up to 62.5-, 63-, 1,553- and 5,215-fold, respectively. Based on the second study, estimated LD50 values of lambda- cyhalothrin for the susceptible and resistant strains were 0.0015 and 0.11 μg a.i. larva-1 , respectively, representing a resistance ratio (RR) of 73.3-fold. The LD50 values of heterozygous were 0.016 and 0.017 μg a.i. larva-1 , indicating an autosomal inheritance of resistance. On lambda-cyhalothrin sprayed-leaves, the resistant strain showed > 85% survival. By contrast, heterozygous and susceptible strains had < 64 and 8% survival, respectively, indicating an incompletely dominant resistance at the field dose of lambda-cyhalothrin. The resistant strain also showed cross-resistance to deltamethrin (RR = 6.2-fold) and cypermethrin (RR = 22.5- fold). Fitness costs studies showed that resistant insects had 71 and 48% lower survival until adult and reproductive rates, respectively, when compared to the Sus strain. Thus, the selection of a C. includens strain resistant to lambda-cyhalothrin showed that resistance inheritance patterns were autosomal, incompletely dominant and associated with substantial fitness costs. The results presented here support the IPM and IRM programs for C. includens in Brazil. Chrysodeixis includens (Walker, 1858) (Lepidoptera: Noctuidae) é uma importante espécie- praga da soja e do algodão no Brasil. O uso de inseticidas é uma das táticas de controle utilizada para essa praga. Visando subsidiar os programas de Manejo Integrado de Pragas (MIP) e o Manejo de Resistência a Insetos (MRI), no primeiro estudo nós caracterizamos a suscetibilidade das populações brasileiras de C. includens a inseticidas e, no segundo estudo, nós selecionamos uma linhagem resistente à lambda-cialotrina e avaliamos a herança da resistência, o custo adaptativo e a resistência cruzada a outros piretroides. No primeiro estudo, populações de campo de C. includens foram coletadas em áreas de soja durante as safras de 2016-2017 e 2017- 2018. No laboratório, larvas L2/L3 foram expostas a inseticidas aplicados sobre a dieta ou em bioensaio com aplicação tópica. Populações de campo de C. includens apresentaram níveis baixos de resistência aos inseticidas espinetoram (CL50 = 0,074 a 0,25 μg i.a. cm-2 ), indoxacarbe (CL50 = 0,46 a 0,94 μg i.a. cm-2 ), tiodicarbe (CL50 = 9,14 a 36,61 μg i.a. cm-2 ), clorantraniliprole (CL50 = 0,15 a 0,57 μg i.a. cm-2 ), flubendiamide (CL50 = 0,45 a 2,01 μg i.a. cm-2 ) e clorfenapir (CL50 = 0,15 a 0,25 μg i.a. cm-2 ); com Razão de Resistência (RR) inferior a 16 vezes. Em contraste, as populações de C. includens apresentaram níveis elevados de resistência à lambda- cialotrina (CL50 = 3,71 a 9,54 μg i.a. cm-2 ), metoxifenozide (CL50 = 0,67 a 4,23 μg i.a. cm-2 ), novaluron (CL50 = 27,52 a 77,63 μg i.a. cm-2 ) e teflubenzuron (CL50 = 13,41 a 73,02 μg i.a. cm- 2 ). Para estes inseticidas a RR foi de 62,5, 63, 1.553 e 5.215 vezes, respectivamente. No segundo estudo, os valores estimados de DL50 para lambda-cialotrina das linhagens suscetível e resistente foram de 0,0015 e 0,11 μg i.a. larva-1 , respectivamente, representando uma RR de 73,3 vezes. Os valores de DL50 dos heterozigotos foram 0,016 e 0,017 µg i.a. larva-1 , indicando uma herança autossômica da resistência. Nos ensaios com folhas de soja pulverizadas com o inseticida lambda-cialotrina, a linhagem resistente apresentou sobrevivência > 85%. Por outro lado, linhagens heterozigotas e suscetíveis tiveram sobrevivência
Databáze:	OpenAIRE
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