Estudo morfoanat?mico e qu?mico de Microlicia hatschbachii Wurdak (Melastomataceae)
| Autor: | Cassiano, Dayse Santos Almeida |
|---|---|
| Přispěvatelé: | Branco, Alexsandro |
| Jazyk: | portugalština |
| Rok vydání: | 2010 |
| Předmět: | |
| Zdroj: | Biblioteca Digital de Teses e Dissertações da UEFS Universidade Estadual de Feira de Santana (UEFS) instacron:UEFS |
| Popis: | Submitted by Bruno Matos Nascimento (brunomatos@uefs.br) on 2020-06-17T18:51:50Z No. of bitstreams: 1 Disserta??o Dayse_final.pdf: 9644271 bytes, checksum: e45708f6c4637d7e9afd4a603a8c0609 (MD5) Made available in DSpace on 2020-06-17T18:51:50Z (GMT). No. of bitstreams: 1 Disserta??o Dayse_final.pdf: 9644271 bytes, checksum: e45708f6c4637d7e9afd4a603a8c0609 (MD5) Previous issue date: 2010-03-03 Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES Microlicia hatschbachii Wurdack belongs to Melastomataceae family,Microlicieae trib. Studies about anatomical and chemical features of Microlicia genus are scarce. This work aimed to describe morpho-anatomy of leaves and stem and to study chemical composition of external constituents of leaf (wax and glandular exudates) and hexane extract of aereal parts of M. hatschbachii. The analysis of wax and glandular exudates was obtained by GC-MS and HPLC-DAD, respectively. The hexane extracts was fractionated by column chromatography using silica gel and the compounds obtained were analyzed by GC-MS. The species has elliptic to oval-elliptic leaves, entire margin, acute apex and rounded symmetrical base. The leaf blade is amphistomatical with anisocytic and anomocytic stomata, the epidermis is unisseriate with isodiametric cells and several glandular trichomes. The mesophyll is isobilateral with bicollateral vascular bundles surrounded by some sclerenchymatic cells. The stem has unisseriated epidermis and bicollateral vascular organization. There are phenolic compounds in epidermical cells and several prisms of calcium oxalate were found in leaves, peciole and stem. Gas chromatographymass spectrometry of the wax shown presence of alkanes and diterpenoids and the analysis of glandular exudates by HPLC-DAD shown the presence of a flavonoid and benzene derivatives. From hexane extract of aereal parts of M. hatschbachii were identified aliphatic alkanes, methyl esters of long chain acids, carboxylic acids and it were isolated for the first time in Melastomataceae the diterpenoids labd 8(17)-en-15-oic acid and labd-8(17),13-dien-15-oic acid. These diterpenoids were identified by 1H and 13C NMR and 2D NMR (COSY, HMQC, HMBC) and other compounds were characterized by GC-MS. Microlicia hatschbachii Wurdack pertence ? fam?lia Melastomataceae, tribo Microlicieae. Estudos sobre as caracter?sticas anat?micas e qu?micas de esp?cies do g?nero Microlicia s?o escassos, assim, este trabalho teve como objetivo a descri??o da morfoanatomia de folhas e caule, o estudo qu?mico dos constituintes externos das folhas (cera e secre??o glandular) e do extrato hex?nico das partes a?reas de M. hatschbachii. A an?lise da cera e do material secretado pelas gl?ndulas foi obtida por CGAR-EM e CLAE-DAD, respectivamente. O extrato hex?nico foi fracionado em coluna cromatogr?fica utilizando-se gel de s?lica e as subst?ncias obtidas foram analisadas por CGAR EM. A esp?cie possui folhas el?pticas a oval-el?pticas, margem inteira, ?pice agudo e base sim?trica e arredondada. A l?mina foliar ? anfistom?tica, com est?matos do tipo anisioc?tico e anomoc?tico, a epiderme ? uniestratificada, com c?lulas isodiam?tricas e presen?a de in?meros tricomas glandulares. O mesofilo ? do tipo isobilateral com feixes vasculares bicolaterais envolvidos por algumas c?lulas esclerenquim?ticas. O caule apresenta epiderme uniestratificada e organiza??o vascular bicolateral. Subst?ncias fen?licas est?o presentes principalmente nas c?lulas epid?rmicas e muitos cristais de oxalato de c?lcio foram encontrados nas folhas, pec?olo e caule. O cromatograma por CG-EM da cera indicou a presen?a de alcanos e diterpenos e a an?lise por CLAE-DAD do material glandular evidenciou a presen?a de um flavon?ide e derivados benz?nicos. A partir do extrato hex?nico das partes a?reas de M. hatschbachii foram identificados alcanos alif?ticos, ?steres met?licos de ?cidos de cadeia longa e ?cidos carbox?licos e foram isolados pela primeira vez em Melastomataceae os diterpenos ?cido labd-8(17)-en-15-?ico e ?cido labd-8(17),13-dien-15-?ico. Estes diterpenos foram identificados por RMN uni (1H e 13C) e bidimensional (COSY, HMQC, HMBC) e as demais subst?ncias foram caracterizadas por CG-EM. |
| Databáze: | OpenAIRE |
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