Effect of elicitor substances on the secondary metabolites accumulatiuon in young plants of Picrolemma sprucei Hook f. (SIMAROUBACEAE), an Amazonian medicinal specie used against malaria

Autor: Silva, Carlos Frederico Nogueira
Přispěvatelé: jonny.pereira@embrapa.br, Pereira, Jonny Everson Scherwinski, Nagao, Eduardo Ossamu, Pohlit, Adrian Martin, Veiga Junior, Valdir Florencio da, Pinto, Ana Cristina da Silva, Melo, M??rcia R??bia Silva, Silva, Simone da
Jazyk: portugalština
Rok vydání: 2009
Předmět:
Zdroj: Biblioteca Digital de Teses e Dissertações da UFAM
Universidade Federal do Amazonas (UFAM)
instacron:UFAM
Popis: Submitted by Carlos Silva (cf.nogueira@yahoo.com.br) on 2019-06-12T23:02:42Z No. of bitstreams: 4 Ata de defesa de tese_doutorado.pdf: 208695 bytes, checksum: b5d166a4278ec227284212002fd9a803 (MD5) carta de encaminhamento.pdf: 546837 bytes, checksum: 0dc4b33f0eef25e4cbae6b0510dc490f (MD5) Tese completa e revisada.pdf: 2167536 bytes, checksum: c7ede04ae0586a610ee98426d4401668 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Approved for entry into archive by PPGBIOTEC Biotecnologia (ppg_biotec.ufam@yahoo.com.br) on 2019-06-13T12:51:38Z (GMT) No. of bitstreams: 4 Ata de defesa de tese_doutorado.pdf: 208695 bytes, checksum: b5d166a4278ec227284212002fd9a803 (MD5) carta de encaminhamento.pdf: 546837 bytes, checksum: 0dc4b33f0eef25e4cbae6b0510dc490f (MD5) Tese completa e revisada.pdf: 2167536 bytes, checksum: c7ede04ae0586a610ee98426d4401668 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Rejected by Divis??o de Documenta????o/BC Biblioteca Central (ddbc@ufam.edu.br), reason: Prezado, Refazer ficha catalogr??fica. 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Aguardamos o envio com as corre????es solicitadas. on 2019-06-13T18:41:35Z (GMT) Submitted by Carlos Silva (cf.nogueira@yahoo.com.br) on 2019-08-12T14:52:55Z No. of bitstreams: 4 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Ata de defesa de tese_doutorado.pdf: 208695 bytes, checksum: b5d166a4278ec227284212002fd9a803 (MD5) carta de encaminhamento.pdf: 546837 bytes, checksum: 0dc4b33f0eef25e4cbae6b0510dc490f (MD5) Tese final.pdf: 2239416 bytes, checksum: b79fbcc199ae6d041987ec009ddddc55 (MD5) Approved for entry into archive by PPGBIOTEC Biotecnologia (ppg_biotec.ufam@yahoo.com.br) on 2019-08-12T20:11:31Z (GMT) No. of bitstreams: 4 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Ata de defesa de tese_doutorado.pdf: 208695 bytes, checksum: b5d166a4278ec227284212002fd9a803 (MD5) carta de encaminhamento.pdf: 546837 bytes, checksum: 0dc4b33f0eef25e4cbae6b0510dc490f (MD5) Tese final.pdf: 2239416 bytes, checksum: b79fbcc199ae6d041987ec009ddddc55 (MD5) Approved for entry into archive by Divis??o de Documenta????o/BC Biblioteca Central (ddbc@ufam.edu.br) on 2019-08-14T13:55:12Z (GMT) No. of bitstreams: 4 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Ata de defesa de tese_doutorado.pdf: 208695 bytes, checksum: b5d166a4278ec227284212002fd9a803 (MD5) carta de encaminhamento.pdf: 546837 bytes, checksum: 0dc4b33f0eef25e4cbae6b0510dc490f (MD5) Tese final.pdf: 2239416 bytes, checksum: b79fbcc199ae6d041987ec009ddddc55 (MD5) Made available in DSpace on 2019-08-14T13:55:12Z (GMT). No. of bitstreams: 4 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Ata de defesa de tese_doutorado.pdf: 208695 bytes, checksum: b5d166a4278ec227284212002fd9a803 (MD5) carta de encaminhamento.pdf: 546837 bytes, checksum: 0dc4b33f0eef25e4cbae6b0510dc490f (MD5) Tese final.pdf: 2239416 bytes, checksum: b79fbcc199ae6d041987ec009ddddc55 (MD5) Previous issue date: 2009-08-28 FAPEAM - Funda????o de Amparo ?? Pesquisa do Estado do Amazonas (61) 99660-6769 The human malaria, one of the most debilitating diseases in the world, remains endemic in tropical regions despite intensive control programs in an attempt to combat it. The increasing spread of antimalarial drug resistance became necessary new drugs against the disease. Picrolemma sprucei Hook. f. (synonym P. pseudocoffea Ducke) is a native shrub and an important medicinal plant from the Amazon Region. The plant has been used in traditional medicine to treat malaria. Its main chemical compounds, the quassinoids isobrucein B and neosergeolide, have presented high in vitro and in vivo antiplasmodial activity. Nevertheless, it??s not know about the production seasonality of these metabolites by the plant, nor the development stage that the plant start to produce them. This study quantified and compared the production of the quassinoids isobrucein B and neosergeolide in P. sprucei plants of in vitro germination, in natural populations of young plants and in green house seedlings maintained in elicitation with methyl jasmonate, methyl salicylate and salicylic acid for 24, 48 and 72 hours, using High Performance Liquid Chromatography, Thin Layer Chromatography and Densitometry. The results showed that in vitro seedlings, after 120 days, presented no detectable quantities of quassinoids. The natural young plants population presented different amounts of quassinoids (up to 0.95% for isobrucein B and 2.37% for neosergeolide) in different parts of the plant. The methyl salicylate has shown to be the best tested elicitor, stimulating an accumulation up to 5.37% of isobrucein B in roots and up to 9.28% of neosergeolide in leaves. Keywords: biotechnology; tissue culture; quassinoids dosages; in vivo secondary metabolites stimulation; isobrucein B; neosergolide. A mal??ria humana, uma das mais debilitantes doen??as parasit??rias do mundo, continua end??mica nas regi??es tropicais apesar dos intensos programas de controle na tentativa de combate-la. A atual resist??ncia ???multi-droga??? do Plasmodium falciparum, respons??vel pela forma mais severa da doen??a, aos antimal??ricos mais eficazes j?? utilizados contra o parasita, torna necess??rio mais e melhores f??rmacos contra a doen??a. Picrolemma sprucei Hook. f. (sinon??mia P. pseudocoffea Ducke) ?? um arbusto nativo e importante planta medicinal da Amaz??nia. Bem conhecida por popula????es ind??genas, tem sido usada h?? bastante tempo na medicina tradicional para tratar mal??ria. Seus principais componentes qu??micos, a isobruce??na B e neosergeolida, da classe dos quassin??ides, apresentam elevada atividade antiplasm??dica in vitro e in vivo. Apesar disso, n??o se conhece sobre a sazonalidade da produ????o desses metab??litos na planta, tampouco em que fase de desenvolvimento a planta come??a a produzi-los. Assim, este trabalho determinou e comparou a produ????o dos quassin??ides isobruce??na B e neosergeolida em plantas de P. sprucei germinadas e cultivadas in vitro, em plantas jovens de popula????es naturais e em plantas jovens mantidas em viveiro e elicitadas com jasmonato de metila, salicilato de metila e ??cido salic??lico por 24, 48 e 72 horas, utilizando Cromatografia L??quida de Alta Efici??ncia, Cromatografia em Camada Delgada e Densitometria. Os resultados mostraram que as pl??ntulas germinadas e cultivadas in vitro, ap??s 120 dias, n??o apresentaram os quassin??ides em n??veis detect??veis. J?? as plantas jovens de popula????es naturais apresentaram ambos em quantidades variadas (at?? 0,95% para isobruce??na e 2,37% para neosergeolida) e diferentes propor????es entre as diferentes partes da planta avaliados. O salicilato de metila se mostrou ser o melhor elicitador testado, estimulando um ac??mulo de at?? 5,37% de isobruce??na B em ra??zes e de at?? 9,28% de neosergeolida em folhas.
Databáze: OpenAIRE
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