The use of the circulating cathodic antigen (Cca) urine cassette assay for the diagnosis and assessment of cure of schistosoma mansoni infections in an endemic

Autor: Sousa, Sergei Rodrigo Magalh?es de, Nogueira, Joyce Favacho Cardoso, Dias, Isabelle Helena Lima, Fonseca, ?lvaro Luan Santana, Favero, Vivian, Geiger, Stefan Michael, Enk, Martin Johannes
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: Repositório Digital do Instituto Evandro Chagas (Patuá)
Instituto Evandro Chagas (IEC)
instacron:IEC
Popis: Universidade do Estado do Par?. Programa de P?s-Gradua??o Strictu Sensu em Biologia Parasit?ria na Amaz?nia. Bel?m, PA, Brasil / Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Laborat?rio de Parasitoses Intestinais, Esquistossomose e Malacologia. Ananindeua, PA, Brasil. Universidade do Estado do Par?. Programa de P?s-Gradua??o Strictu Sensu em Biologia Parasit?ria na Amaz?nia. Bel?m, PA, Brasil / Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Laborat?rio de Parasitoses Intestinais, Esquistossomose e Malacologia. Ananindeua, PA, Brasil. Universidade do Estado do Par?. Programa de P?s-Gradua??o Strictu Sensu em Biologia Parasit?ria na Amaz?nia. Bel?m, PA, Brasil / Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Laborat?rio de Parasitoses Intestinais, Esquistossomose e Malacologia. Ananindeua, PA, Brasil. Universidade do Estado do Par?. Programa de P?s-Gradua??o Strictu Sensu em Biologia Parasit?ria na Amaz?nia. Bel?m, PA, Brasil / Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Laborat?rio de Parasitoses Intestinais, Esquistossomose e Malacologia. Ananindeua, PA, Brasil. Pontif?cia Universidade Cat?lica do Rio Grande do Sul. Programa de P?s-Gradua??o em Medicina e Ci?ncias da Sa?de. Laborat?rio de Parasitologia Biom?dica. Porto Alegre, RS, Brasil. Universidade Federal de Minas Gerais. Departamento de Parasitologia. Belo Horizonte, MG, Brasil. Universidade do Estado do Par?. Programa de P?s-Gradua??o Strictu Sensu em Biologia Parasit?ria na Amaz?nia. Bel?m, PA, Brasil / Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Laborat?rio de Parasitoses Intestinais, Esquistossomose e Malacologia. Ananindeua, PA, Brasil. INTRODUCTION: Schistosomiasis is a poverty-related disease that affects people in 78 countries worldwide. This study aimed to evaluate the point-of-care circulating cathodic antigen (POC-CCA) test performance using sensitive parasitological methods as a reference standard (RS) in individuals before and after treatment. METHODS: The RS was established by combining the results of 16 Kato-Katz slides and the Helmintex? method. Positivity rates of the POC-CCA test and Kato-Katz and Helmintex? methods were calculated before treatment and 30 days afterward. Furthermore, the sensitivity, specificity, accuracy, and kappa coefficient before treatment were determined by comparing the methods. The cure rate was defined 30 days after treatment. RESULTS: Among the 217 participants, the RS detected a total of 63 (29.0%) positive individuals. The POC-CCA test identified 79 (36.4%) infections. The evaluation of POC-CCA test performance in relation to the RS revealed a sensitivity of 61.9%, specificity of 74.0%, accuracy of 70.5%, and kappa coefficient of 0.33. Out of the 53 remaining participants after treatment, a total of 45 (81.1%) showed egg negative results, and 8 (18.9%) were egg positive according to the RS. A total of 5 (9.4%) egg-positive and 37 (69.8%) egg-negative individuals were positive by the POC-CCA test. CONCLUSIONS: Our data show that the POC-CCA test has potential as an auxiliary tool for the diagnosis of Schistosoma mansoni infection, yielding better results than 16 Kato-Katz slides from three different stool samples. However, the immunochromatographic test lacks sufficient specificity and sensitivity for verifying the cure rate after treatment.
Databáze: OpenAIRE
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