Experimental Parasitology

Autor: Freitas, Luiz Antônio Rodrigues de, Santana, Cláudia Dias de, Veras, Patrícia Sampaio Tavares, Welby-Borges, Marcus, Nihei, Jorge S., Cardillo, Fabíola
Jazyk: angličtina
Rok vydání: 2006
Předmět:
Zdroj: Repositório Institucional da UFBA
Universidade Federal da Bahia (UFBA)
instacron:UFBA
ISSN: 0014-4894
DOI: 10.1016/j.exppara.2006.01.008
Popis: Texto completo: acesso restrito. p.201–205 Submitted by Edileide Reis (leyde-landy@hotmail.com) on 2013-08-08T12:11:07Z No. of bitstreams: 1 1-s2.0-S0014489406000099-main.pdf: 304340 bytes, checksum: 057e83846ed49fde782d133e5d48ac0a (MD5) Made available in DSpace on 2013-08-08T12:11:07Z (GMT). No. of bitstreams: 1 1-s2.0-S0014489406000099-main.pdf: 304340 bytes, checksum: 057e83846ed49fde782d133e5d48ac0a (MD5) Previous issue date: 2006 CBA/J mice are resistant to Leishmania major and susceptible to Leishmania amazonensis. Early events determine infection outcome. Until now, PIV (in vitro priming) immune response to L. amazonensis has not been assessed. Herein, we have shown that compared to L. major, L. amazonensis induced higher parasite burden associated to similar IL-4, IFN-γ, and TNF-α mRNA expressions and IFN-γ and IL-10 levels. Although similar amounts of IL-10 were detected, the frequency of intracellular IL-10 positive B cells was enhanced in spleen cells stimulated with anti-CD3/anti-CD28, or anti-CD3/anti-CD28 and L. amazonensis, compared to L. major-stimulation. Interestingly, IL-10- producing B cells were reduced in response to anti-CD3/anti-CD28 stimulation combined with L. major compared to the other groups. L. amazonensis may favor T regulatory cell development, since 40% of all the CD4+CD25+ were CD25high cells. These data suggest that in PIV, susceptibility to L. amazonensis is not related to Th cell polarization, but to the presence and activity of regulatory T and B cells.
Databáze: OpenAIRE
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