Journal of Chromatographic Science
| Autor: | Silva, Vilma Mota da, Veloso, Márcia Cristina da Cunha, Sousa, Eliane Teixeira, Santos, Gislaine Vieira dos, Accioly, Miguel da Costa, Pereira, Pedro Afonso de Paula, Andrade, Jailson Bittencourt de |
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| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Zdroj: | Repositório Institucional da UFBA Universidade Federal da Bahia (UFBA) instacron:UFBA |
| DOI: | 10.1093/chromsci/44.5.233 |
| Popis: | Texto completo: acesso restrito. p.233-238 Submitted by Edileide Reis (leyde-landy@hotmail.com) on 2013-07-04T14:04:03Z No. of bitstreams: 1 233.full.pdf: 160550 bytes, checksum: 3642121710f8e8152bbc86ed34cf9cec (MD5) Made available in DSpace on 2013-07-04T14:04:03Z (GMT). No. of bitstreams: 1 233.full.pdf: 160550 bytes, checksum: 3642121710f8e8152bbc86ed34cf9cec (MD5) Previous issue date: 2006 A new analytical method is reported for the determination of 11 volatile carbonyl compounds isolated at room temperature from the headspace of marine algae. This method is based on the conversion of the carbonyl compounds to their 2,4-dinitrophenylhydrazone derivatives followed by high-performance liquid chromatography analysis. Using this method, 11 carbonyl compounds are detected and identified from the dynamic headspace sampling of 10 species of marine algae. Eight compounds are quantitated and the three remaining are only identified. Under optimized conditions, all carbonyl compounds are separated in 32 min. The detection and quantitation limits of the high-performance liquid chromatography method are, respectively, in the range of 0.26–0.85 ng/g of algae (formaldehyde) to 13.77–45.90 ng/g of algae (E )-2-hexenal. The calibration curves are linear in the concentration range of 2.0–1000 µg/L of solution, corresponding to 0.34–170.00 ng/g of algae. Acetaldehyde and propanal are the most abundant carbonyl compounds identified, with concentrations as high as 980 and 790 ng/g, respectively. The present work, as far as we know, is the first analytical methodology that has been developed to determine low-molecular-weight carbonyl compounds in marine algae. Because many species of marine algae are used as human food, the reported method should be useful to investigators studying the nutritional value as well as oxidative spoilage of fresh and preserved marine algae that is destined for human consumption. |
| Databáze: | OpenAIRE |
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