BtaE, an adhesin that belongs to the trimeric autotransporter family, is required for full virulence and defines a specific adhesive pole of Brucella suis
| Autor: | Ruiz-Ranwez, V., Posadas, D.M., Van der Henst, C., Estein, S.M., Arocena, G.M., Abdian, P.L., Martín, F.A., Sieira, R., De Bolle, X., Zorreguieta, A. |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
in vitro study
Brucella suis phenotype protein binding protein localization Bacterial Adhesion Brucellosis in vivo study Mice adhesin fibronectin hyaluronic acid Escherichia coli Animals Adhesins Bacterial protein expression Yersinia enterocolitica btae protein Mice Inbred BALB C Virulence bacterial virulence article Cell Polarity Gene Expression Regulation Bacterial protein function bacterial strain bacterium adherence Antibodies Bacterial unclassified drug internalization cell surface priority journal Multigene Family computer model protein epithelium cell Carrier Proteins |
| Zdroj: | Infect. Immun. 2013;81(3):996-1007 Biblioteca Digital (UBA-FCEN) Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales instacron:UBA-FCEN |
| Popis: | Brucella is responsible for brucellosis, one of the most common zoonoses worldwide that causes important economic losses in several countries. Increasing evidence indicates that adhesion of Brucella spp. to host cells is an important step to establish infection. We have previously shown that the BmaC unipolar monomeric autotransporter mediates the binding of Brucella suis to host cells through cell-associated fibronectin. Our genome analysis shows that the B. suis genome encodes several additional potential adhesins. In this work, we characterized a predicted trimeric autotransporter that we named BtaE. By expressing btaE in a nonadherent Escherichia coli strain and by phenotypic characterization of a B. suis δbtaE mutant, we showed that BtaE is involved in the binding of B. suis to hyaluronic acid. The B. suis δbtaE mutant exhibited a reduction in the adhesion to HeLa and A549 epithelial cells compared with the wild-type strain, and it was outcompeted by the wild-type strain in the binding to HeLa cells. The knockout btaE mutant showed an attenuated phenotype in the mouse model, indicating that BtaE is required for full virulence. BtaE was immunodetected on the bacterial surface at one cell pole. Using old and new pole markers, we observed that both the BmaC and BtaE adhesins are consistently associated with the new cell pole, suggesting that, in Brucella, the new pole is functionally differentiated for adhesion. This is consistent with the inherent polarization of this bacterium, and its role in the invasion process. © 2013, American Society for Microbiology. Fil:Posadas, D.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Abdian, P.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Martín, F.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Zorreguieta, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. |
| Databáze: | OpenAIRE |
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