Ecotoxicological evaluation of water from the Sorocaba River using an integrated analysis of biochemical and morphological biomarkers in bullfrog tadpoles, Lithobates catesbeianus (Shaw, 1802)

Autor: Fernandes, Isabela Ferreira, Utsunomiya, Heidi Samantha Moraes, de Lacerda Valverde, Bruno Serra [UNESP], Ferraz, João Victor Cassiel, Fujiwara, Gabriel Hiroshi, Gutierres, Davi Marques, de Oliveira, Classius [UNESP], Belussi, Lilian Franco, Fernandes, Marisa Narciso, Carvalho, Cleoni dos Santos
Přispěvatelé: Universidade Federal de São Carlos (UFSCar), Universidade Estadual Paulista (Unesp), Universidade Federal de Mato Grosso do Sul (UFMS)
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: Scopus
Repositório Institucional da UNESP
Universidade Estadual Paulista (UNESP)
instacron:UNESP
Popis: Made available in DSpace on 2021-06-25T10:54:12Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-07-01 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Lithobates catesbeianus tadpoles were exposed for 96 h to water from two sites of the Sorocaba River (summer and winter), Ibiúna (PI) and Itupararanga reservoir (PIR) that contained metals. In the liver, in PI, the glutathione peroxidase (GPx) decreased, and the glutathione S-transferase (GST) and carbonyl proteins (PCO) increased. In PIR, the glutathione reduced (GSH) increased, while there was a decrease in catalase (CAT), GPx, GST, PCO, and superoxide dismutase (SOD). In winter, GPx and GST increased in both points. Regarding the kidneys, lipoperoxidation (LPO) levels and GST decreased, while GSH increased in the summer. In the winter, LPO increased in PI. In the muscle, in the summer, there was an increase in GSH and GST and change in PCO. In the winter, the levels of PCO increased and CAT decreased in PIR. The area and volume of the hepatocyte and nucleus area increased in the summer and decreased in the winter. Hepatic melanin decreased in the summer after exposure to PIR water. There were the systemic effects of Sorocaba River water exposure at different times of the year with alterations in biomarkers at different levels, in which kidney shows highest Integrated Response of Biomarkers (IBR) value followed by liver and muscle. Biochemical biomarkers were more sensitive than morphological ones. The more sensitive biochemical markers were MT, PCO, GST and LPO. These effects confirm the hypothesis of metabolic alteration in bullfrog tadpoles by the Sorocaba River water. Acute exposure to water from the Sorocaba River induced oxidative stress, mainly in the liver and kidneys, and caused liver damage in bullfrog tadpoles even in low concentrations of metals. Programa de Pós-graduação Em Biotecnologia e Monitoramento Ambiental Universidade Federal de São Carlos Campus Sorocaba, Rodovia João Leme Dos Santos, Km 110, SP-264 Universidade Federal de São Carlos Campus Sorocaba Laboratório de Bioquímica e Microbiologia (LaBioM), Rodovia João Leme Dos Santos, Km 110, SP-264 Universidade Estadual Paulista Programa de Pós-Graduação Em Biologia Animal da UNESP, Campus de São José Do Rio Preto Departamento de Biologia Universidade Estadual Paulista Universidade Federal de Mato Grosso Do Sul Instituto de Biociências Laboratório de Patologia Experimental (LAPex) Universidade Federal de São Carlos Departamento de Ciências Fisiológicas, Caixa Postal 676, Rodovia Washington Luís Km 235 Universidade Estadual Paulista Programa de Pós-Graduação Em Biologia Animal da UNESP, Campus de São José Do Rio Preto Departamento de Biologia Universidade Estadual Paulista FAPESP: 2017/23781-9 CNPq: 304552/2019-4
Databáze: OpenAIRE
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