Fungal proteases: production using soybean bran and banana flours, enzymatic characterization and application in cricket flour

Autor: Koike, Meliane Akemi
Přispěvatelé: Universidade Estadual Paulista (Unesp), Fleuri, Luciana Francisco [UNESP], Bagagli, Marcela Pavan
Jazyk: portugalština
Rok vydání: 2020
Předmět:
Zdroj: Repositório Institucional da UNESP
Universidade Estadual Paulista (UNESP)
instacron:UNESP
Popis: Submitted by Meliane Akemi Koike (melkoike@gmail.com) on 2020-05-08T15:39:07Z No. of bitstreams: 1 Dissertação Final Mel.pdf: 4897180 bytes, checksum: 4452b950f320e3a2f8db4ba4232f7efd (MD5) Submitted by Meliane Akemi Koike (melkoike@gmail.com) on 2020-05-08T15:39:07Z No. of bitstreams: 1 Dissertação Final Mel.pdf: 4897180 bytes, checksum: 4452b950f320e3a2f8db4ba4232f7efd (MD5) Submitted by Meliane Akemi Koike (melkoike@gmail.com) on 2020-05-08T15:39:07Z No. of bitstreams: 1 Dissertação Final Mel.pdf: 4897180 bytes, checksum: 4452b950f320e3a2f8db4ba4232f7efd (MD5) Approved for entry into archive by ROSANGELA APARECIDA LOBO null (rosangelalobo@btu.unesp.br) on 2020-05-11T13:55:31Z (GMT) No. of bitstreams: 1 koike_ma_me_bot_par.pdf: 1080407 bytes, checksum: 0b7e4fbc59ff2cf8c7de06eb8f53a7ed (MD5) Approved for entry into archive by ROSANGELA APARECIDA LOBO null (rosangelalobo@btu.unesp.br) on 2020-05-11T13:55:31Z (GMT) No. of bitstreams: 1 koike_ma_me_bot_par.pdf: 1080407 bytes, checksum: 0b7e4fbc59ff2cf8c7de06eb8f53a7ed (MD5) Submitted by Meliane Akemi Koike (melkoike@gmail.com) on 2020-05-08T15:39:07Z No. of bitstreams: 1 Dissertação Final Mel.pdf: 4897180 bytes, checksum: 4452b950f320e3a2f8db4ba4232f7efd (MD5) Approved for entry into archive by ROSANGELA APARECIDA LOBO null (rosangelalobo@btu.unesp.br) on 2020-05-11T13:55:31Z (GMT) No. of bitstreams: 1 koike_ma_me_bot_par.pdf: 1080407 bytes, checksum: 0b7e4fbc59ff2cf8c7de06eb8f53a7ed (MD5) Submitted by Meliane Akemi Koike (melkoike@gmail.com) on 2020-05-08T15:39:07Z No. of bitstreams: 1 Dissertação Final Mel.pdf: 4897180 bytes, checksum: 4452b950f320e3a2f8db4ba4232f7efd (MD5) Approved for entry into archive by ROSANGELA APARECIDA LOBO null (rosangelalobo@btu.unesp.br) on 2020-05-11T13:55:31Z (GMT) No. of bitstreams: 1 koike_ma_me_bot_par.pdf: 1080407 bytes, checksum: 0b7e4fbc59ff2cf8c7de06eb8f53a7ed (MD5) Made available in DSpace on 2020-05-11T13:55:31Z (GMT). No. of bitstreams: 1 koike_ma_me_bot_par.pdf: 1080407 bytes, checksum: 0b7e4fbc59ff2cf8c7de06eb8f53a7ed (MD5) Previous issue date: 2020-03-24 Made available in DSpace on 2020-05-11T13:55:31Z (GMT). No. of bitstreams: 1 koike_ma_me_bot_par.pdf: 1080407 bytes, checksum: 0b7e4fbc59ff2cf8c7de06eb8f53a7ed (MD5) Previous issue date: 2020-03-24 Approved for entry into archive by ROSANGELA APARECIDA LOBO null (rosangelalobo@btu.unesp.br) on 2020-05-11T13:55:31Z (GMT) No. of bitstreams: 1 koike_ma_me_bot_par.pdf: 1080407 bytes, checksum: 0b7e4fbc59ff2cf8c7de06eb8f53a7ed (MD5) Approved for entry into archive by ROSANGELA APARECIDA LOBO null (rosangelalobo@btu.unesp.br) on 2020-05-11T13:55:31Z (GMT) No. of bitstreams: 1 koike_ma_me_bot_par.pdf: 1080407 bytes, checksum: 0b7e4fbc59ff2cf8c7de06eb8f53a7ed (MD5) Made available in DSpace on 2020-05-11T13:55:31Z (GMT). No. of bitstreams: 1 koike_ma_me_bot_par.pdf: 1080407 bytes, checksum: 0b7e4fbc59ff2cf8c7de06eb8f53a7ed (MD5) Previous issue date: 2020-03-24 Submitted by Meliane Akemi Koike (melkoike@gmail.com) on 2020-05-08T15:39:07Z No. of bitstreams: 1 Dissertação Final Mel.pdf: 4897180 bytes, checksum: 4452b950f320e3a2f8db4ba4232f7efd (MD5) Approved for entry into archive by ROSANGELA APARECIDA LOBO null (rosangelalobo@btu.unesp.br) on 2020-05-11T13:55:31Z (GMT) No. of bitstreams: 1 koike_ma_me_bot_par.pdf: 1080407 bytes, checksum: 0b7e4fbc59ff2cf8c7de06eb8f53a7ed (MD5) Made available in DSpace on 2020-05-11T13:55:31Z (GMT). No. of bitstreams: 1 koike_ma_me_bot_par.pdf: 1080407 bytes, checksum: 0b7e4fbc59ff2cf8c7de06eb8f53a7ed (MD5) Previous issue date: 2020-03-24 Submitted by Meliane Akemi Koike (melkoike@gmail.com) on 2020-05-08T15:39:07Z No. of bitstreams: 1 Dissertação Final Mel.pdf: 4897180 bytes, checksum: 4452b950f320e3a2f8db4ba4232f7efd (MD5) Made available in DSpace on 2020-05-11T13:55:31Z (GMT). No. of bitstreams: 1 koike_ma_me_bot_par.pdf: 1080407 bytes, checksum: 0b7e4fbc59ff2cf8c7de06eb8f53a7ed (MD5) Previous issue date: 2020-03-24 Approved for entry into archive by ROSANGELA APARECIDA LOBO null (rosangelalobo@btu.unesp.br) on 2020-05-11T13:55:31Z (GMT) No. of bitstreams: 1 koike_ma_me_bot_par.pdf: 1080407 bytes, checksum: 0b7e4fbc59ff2cf8c7de06eb8f53a7ed (MD5) Made available in DSpace on 2020-05-11T13:55:31Z (GMT). No. of bitstreams: 1 koike_ma_me_bot_par.pdf: 1080407 bytes, checksum: 0b7e4fbc59ff2cf8c7de06eb8f53a7ed (MD5) Previous issue date: 2020-03-24 Approved for entry into archive by ROSANGELA APARECIDA LOBO null (rosangelalobo@btu.unesp.br) on 2020-05-11T13:55:31Z (GMT) No. of bitstreams: 1 koike_ma_me_bot_par.pdf: 1080407 bytes, checksum: 0b7e4fbc59ff2cf8c7de06eb8f53a7ed (MD5) Made available in DSpace on 2020-05-11T13:55:31Z (GMT). No. of bitstreams: 1 koike_ma_me_bot_par.pdf: 1080407 bytes, checksum: 0b7e4fbc59ff2cf8c7de06eb8f53a7ed (MD5) Previous issue date: 2020-03-24 Submitted by Meliane Akemi Koike (melkoike@gmail.com) on 2020-05-08T15:39:07Z No. of bitstreams: 1 Dissertação Final Mel.pdf: 4897180 bytes, checksum: 4452b950f320e3a2f8db4ba4232f7efd (MD5) Approved for entry into archive by ROSANGELA APARECIDA LOBO null (rosangelalobo@btu.unesp.br) on 2020-05-11T13:55:31Z (GMT) No. of bitstreams: 1 koike_ma_me_bot_par.pdf: 1080407 bytes, checksum: 0b7e4fbc59ff2cf8c7de06eb8f53a7ed (MD5) Made available in DSpace on 2020-05-11T13:55:31Z (GMT). No. of bitstreams: 1 koike_ma_me_bot_par.pdf: 1080407 bytes, checksum: 0b7e4fbc59ff2cf8c7de06eb8f53a7ed (MD5) Previous issue date: 2020-03-24 Made available in DSpace on 2020-05-11T13:55:31Z (GMT). No. of bitstreams: 1 koike_ma_me_bot_par.pdf: 1080407 bytes, checksum: 0b7e4fbc59ff2cf8c7de06eb8f53a7ed (MD5) Previous issue date: 2020-03-24 Made available in DSpace on 2020-05-11T13:55:31Z (GMT). No. of bitstreams: 1 koike_ma_me_bot_par.pdf: 1080407 bytes, checksum: 0b7e4fbc59ff2cf8c7de06eb8f53a7ed (MD5) Previous issue date: 2020-03-24 Approved for entry into archive by ROSANGELA APARECIDA LOBO null (rosangelalobo@btu.unesp.br) on 2020-05-11T13:55:31Z (GMT) No. of bitstreams: 1 koike_ma_me_bot_par.pdf: 1080407 bytes, checksum: 0b7e4fbc59ff2cf8c7de06eb8f53a7ed (MD5) Approved for entry into archive by ROSANGELA APARECIDA LOBO null (rosangelalobo@btu.unesp.br) on 2020-05-11T13:55:31Z (GMT) No. of bitstreams: 1 koike_ma_me_bot_par.pdf: 1080407 bytes, checksum: 0b7e4fbc59ff2cf8c7de06eb8f53a7ed (MD5) Made available in DSpace on 2020-05-11T13:55:31Z (GMT). No. of bitstreams: 1 koike_ma_me_bot_par.pdf: 1080407 bytes, checksum: 0b7e4fbc59ff2cf8c7de06eb8f53a7ed (MD5) Previous issue date: 2020-03-24 Approved for entry into archive by ROSANGELA APARECIDA LOBO null (rosangelalobo@btu.unesp.br) on 2020-05-11T13:55:31Z (GMT) No. of bitstreams: 1 koike_ma_me_bot_par.pdf: 1080407 bytes, checksum: 0b7e4fbc59ff2cf8c7de06eb8f53a7ed (MD5) Made available in DSpace on 2020-05-11T13:55:31Z (GMT). No. of bitstreams: 1 koike_ma_me_bot_par.pdf: 1080407 bytes, checksum: 0b7e4fbc59ff2cf8c7de06eb8f53a7ed (MD5) Previous issue date: 2020-03-24 Made available in DSpace on 2020-05-11T13:55:31Z (GMT). No. of bitstreams: 1 koike_ma_me_bot_par.pdf: 1080407 bytes, checksum: 0b7e4fbc59ff2cf8c7de06eb8f53a7ed (MD5) Previous issue date: 2020-03-24 Approved for entry into archive by ROSANGELA APARECIDA LOBO null (rosangelalobo@btu.unesp.br) on 2020-05-11T13:55:31Z (GMT) No. of bitstreams: 1 koike_ma_me_bot_par.pdf: 1080407 bytes, checksum: 0b7e4fbc59ff2cf8c7de06eb8f53a7ed (MD5) Made available in DSpace on 2020-05-11T13:55:31Z (GMT). No. of bitstreams: 1 koike_ma_me_bot_par.pdf: 1080407 bytes, checksum: 0b7e4fbc59ff2cf8c7de06eb8f53a7ed (MD5) Previous issue date: 2020-03-24 Approved for entry into archive by ROSANGELA APARECIDA LOBO null (rosangelalobo@btu.unesp.br) on 2020-05-11T13:55:31Z (GMT) No. of bitstreams: 1 koike_ma_me_bot_par.pdf: 1080407 bytes, checksum: 0b7e4fbc59ff2cf8c7de06eb8f53a7ed (MD5) Made available in DSpace on 2020-05-11T13:55:31Z (GMT). No. of bitstreams: 1 koike_ma_me_bot_par.pdf: 1080407 bytes, checksum: 0b7e4fbc59ff2cf8c7de06eb8f53a7ed (MD5) Previous issue date: 2020-03-24 Made available in DSpace on 2020-05-11T13:55:31Z (GMT). No. of bitstreams: 1 koike_ma_me_bot_par.pdf: 1080407 bytes, checksum: 0b7e4fbc59ff2cf8c7de06eb8f53a7ed (MD5) Previous issue date: 2020-03-24 Resíduos e subprodutos agroindustriais são extensivamente estudados a fim de se reaproveitar seu potencial biológico e agregar valor. A fermentação em estado sólido (FES) é uma das formas de utilizar estes resíduos e subprodutos para obtenção de enzimas, como as proteases. Este trabalho objetivou estudar a produção de proteases fúngicas por FES utilizando farelo de soja e farinhas de banana, caracterizar bioquimicamente as proteases mais promissoras e, por fim, aplicar na hidrólise da farinha de grilo (Gryllus assimilis), avaliando os produtos de hidrólise. O estudo da composição do meio de cultivo foi conduzido através de um delineamento experimental, o planejamento de misturas, para definir a proporção ótima de parte sólida dos substratos de fermentação. Posteriormente, realizou-se a cinética de produção de protease por FES, a caracterização bioquímica das proteases mais promissoras quanto ao pH e temperatura ótimos de atuação, bem como a influência de íons metálicos e L-cisteína em diferentes concentrações. Por meio de relargagem (salting-out), fez-se a purificação parcial das enzimas, seguida de diálise e liofilização, para então serem aplicadas na hidrólise da farinha de grilo. O fator de hidrólise, a atividade antioxidante e o perfil eletroforético dos produtos de hidrólise foram avaliados. O planejamento de misturas resultou em duas proporções com altas atividades proteolíticas, 50 % de farelo de soja (FS) e 50 % de farinha de casca de banana madura (FCBM) e um terço de cada componente estudado (farelo de soja, farinha de casca de banana madura e farinha de banana verde) para os dois micro-organismos estudados: Trichoderma koningii (INCQS 40331) e Aspergillus oryzae (ATCC 1003 / INCQS 40068). Foi possível obter um modelo quadrático apenas para o A. oryzae, com p
Databáze: OpenAIRE
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