Clostridium difficile toxins A and B induce differential adenosine receptors expression in intestinal epithelial cells: the role of A2B adenosine receptor
| Autor: | Foschetti, Danielle Abreu |
|---|---|
| Přispěvatelé: | Brito, Gerly Anne de Castro |
| Jazyk: | portugalština |
| Rok vydání: | 2014 |
| Předmět: | |
| Zdroj: | Repositório Institucional da Universidade Federal do Ceará (UFC) Universidade Federal do Ceará (UFC) instacron:UFC |
| Popis: | Clostridium difficile is recognized to be a nosocomial pathogen that causes intense intestinal inflammation, epithelial barrier disruption and diarrhea. Adenosine production is increased under inflammatory situations. The adenosine receptor A2B is the most expressed receptor in the human and mice intestine. We investigated the effect of short- and long-term exposure to TcdA and TcdB in HCT-8 cells and isolated cecum epithelial cells. HCT-8 cells were exposed to TcdA or TcdB (10 ng/ml) for 2, 6 and 24h. We used a murine cecal loop model and murine infection model to evaluate the effects of TcdA and C. difficile infection, respectively. We demonstrated that HCT-8 and isolated intestinal cecum epithelial cells naturally express high levels of A2BR receptors. TcdA or TcdB alters the cell morphology, viability and proliferation pattern and caused gene expression increase of all AR subtypes in HCT-8. In isolated cecum epithelial cells, TcdA significantly (p |
| Databáze: | OpenAIRE |
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