An a-amylase inhibitor gene from Phaseolus coccineus encodes a protein with potential for control of coffee berry borer (Hypothenemus hampei)
| Autor: | Pereira, Railene de Azevedo, Batista, João Aguiar Nogueira, Silva, Maria Cristina Mattar da, Oliveira Neto, Osmundo Brilhante de, Figueira, Edson Luiz Zangrando, Jiménez, Arnubio Valencia, Grossi-de-Sá, Maria Fátima |
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| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: | |
| Zdroj: | Repositório Institucional da UCB Universidade Católica de Brasília (UCB) instacron:UCB |
| ISSN: | 4764-2971 |
| Popis: | Made available in DSpace on 2016-10-10T03:52:45Z (GMT). No. of bitstreams: 5 An amylase inhibitor gene from Phaseolus coccineus.PDF: 444316 bytes, checksum: f99487a1aef5cbc85d3aa0976bf8eb73 (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 23851 bytes, checksum: 294cb7010cc40c47642971e073de3dba (MD5) license_rdf: 23892 bytes, checksum: afd5dad10b1d1e6dc10c8c5d25222c7a (MD5) license.txt: 1887 bytes, checksum: 445d1980f282ec865917de35a4c622f6 (MD5) Previous issue date: 2006 Plant a-amylase inhibitors are proteins found in several plants, and play a key role in natural defenses. In this study, a gene encoding an a-amylase inhibitor, named aAI-Pc1, was isolated from cotyledons of Phaseolus coccineus. This inhibitor has an enhanced primary structure to P. vulgaris a-amylase inhibitors (a-AI1 and a-AI2). The aAI-Pc1 gene, constructed with the PHA-L phytohemaglutinin promoter, was introduced into tobacco plants, with its expression in regenerated (T0) and progeny (T1) transformant plants monitored by PCR amplification, enzyme-linked immunosorbent assay (ELISA) and immunoblot analysis, respectively. Seed protein extracts from selected transformants reacted positively with a polyclonal antibody raised against aAI-1, while no reaction was observed with untransformed tobacco plants. Immunological assays showed that the aAI-Pc1 gene product represented up to 0.05% of total soluble proteins in T0 plants seeds. Furthermore, recombinant aAI-Pc1 expressed in tobacco plants was able to inhibit 65% of digestive H. hampei a-amylases. The data herein suggest that the protein encoded by the aAI-Pc1 gene has potential to be introduced into coffee plants in order to increase their resistance to the coffee berry borer. Sim Publicado |
| Databáze: | OpenAIRE |
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