Diversity of ejaculated sperm proteins in Moxotó bucks (Capra hircus) evaluated by multiple extraction methods

Autor: MOREIRA, R. F., MATOS, M. N. C., ALVES FILHO, J. G., VALLE, R. V. do, ELOY, A. M. X., PINTO, T. M. F., MACHADO, S. P., COSTA, C. R. R., LIMA FILHO, J. L. de, LIMA, J. P. M. S., CUNHA, R. M. S. da
Přispěvatelé: Raulzito Fernandes Moreira, Pós-graduação - Universidade Federal do Ceará (UFC) - Sobral, CE, Brasil., Tatiana Maria Farias Pinto, Saris Pinto Machado Junior, Cíntia Renata Rocha Costa, José Luiz de Lima Filho, João Paulo Matos Santos Lima, Rodrigo Maranguape Silva da Cunha., João Garcia Alves Filho, ANGELA MARIA XAVIER ELOY, CNPC, Maria Nágila Carneiro Matos, Roberta Vianna do Valle
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Zdroj: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA-Alice)
Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
Popis: This study aimed to develop protocols for the extraction of sperm proteins from Moxotó goats (Capra hircus) and to compare the resulting proteomic maps. The sperm proteins were isolated using an extraction buffer containing 7 M urea and 2 M thiourea, 20 mM DTT, and one of the following detergents: 1% or 4% CHAPS; 1% or 4% SDS; 1% or 4% Triton X-100; or a combination of CHAPS and SDS. The 1-DE and 2-DE profiles of the isolated proteins revealed that the various isolation methods were efficient. Qualitative and quantitative differences in the 1-DE and 2-DE profiles were observed. 2-DE maps indicated that the amount and diversity of proteins visualized depended on the detergent that was used. Furthermore, this work revealed that the combination of detergents increased the resolution of some spots and retained the characteristics of the individual detergents, depending on their concentrations. Made available in DSpace on 2018-04-20T01:08:20Z (GMT). No. of bitstreams: 1 CNPCDiversity2018.pdf: 1187342 bytes, checksum: 4f046c9fafdc2f7d425ef23b24953998 (MD5) Previous issue date: 2018-04-12
Databáze: OpenAIRE
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