Quantitative and qualitative assessment of changes in splenic tissue of mice with schistossomiasis and dyslipidemic

Autor:	Silva, Adriana Matias da
Přispěvatelé:	Silva, José Roberto Machado e, Neves, Renata Heisler, Machado, Ana Carolina Stumbo, Amendoeira, Maria Regina Reis
Jazyk:	portugalština
Rok vydání:	2012
Předmět:	Histopathology. Morphometry. Stereology splenic architecture arquitetura esplênica esplenopatias morfometria hypercholeristemic chow histopatologia dieta hipercoleristêmica CIENCIAS BIOLOGICAS::PARASITOLOGIA [CNPQ] dislipidemia schistosoma mansoni estereologia
Zdroj:	Biblioteca Digital de Teses e Dissertações da UERJ Universidade do Estado do Rio de Janeiro (UERJ) instacron:UERJ
Popis:	Submitted by Boris Flegr (boris@uerj.br) on 2021-01-05T18:13:06Z No. of bitstreams: 2 Pre-textual 240512.pdf: 108054 bytes, checksum: bec373c6b5d3843a038f371d486408cc (MD5) Textual 240512.pdf: 8280166 bytes, checksum: 787e9d540b45c585bdc6389deeff9ddc (MD5) Made available in DSpace on 2021-01-05T18:13:06Z (GMT). No. of bitstreams: 2 Pre-textual 240512.pdf: 108054 bytes, checksum: bec373c6b5d3843a038f371d486408cc (MD5) Textual 240512.pdf: 8280166 bytes, checksum: 787e9d540b45c585bdc6389deeff9ddc (MD5) Previous issue date: 2012-04-27 The effect of the changes promoted by hypercholesterolemia and experimental schistosomiasis infection on splenic architeture has remained elusive. In this study, we investigated the qualitative and quantitative organization of the splenic tissue in the acute phase (ninth week) of Schistosoma mansoni infection of mice fed either a hypercholesterolemic (29% lipids) or a standard chow (12% lipids). Spleen volume was measured by Scherlis Method. Histological sections (5μm) were stained with hematoxylin-eosin, Lennert's Giemsa and Picrosirius. The splenic disorganization was evaluated by histopathology, morphometry and stereology. Our major findings demonstrated that both diet and infection determined perturbations in splenic compartments. Infected mice, regardless of diet, had larger spleen (Student s t-test, P=0.004) and disorganized splenic architecture than in corresponding uninfected mice, for which no histopathology was evident. The white pulp compartment was reduced, whereas red pulp and germinal center were enhanced (Student s t-test, P
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=od______3056::6f53830394d3078431308c5e518bdc3e http://www.bdtd.uerj.br/handle/1/7827 Zobrazit plný text záznamu