Evaluation of in vitro virulence of clinical and environmental isolates of dermatophyte fungi

Autor: Bonci, M?rio Mendes
Přispěvatelé: Baroni, Francisco de Assis, Costa, Gisela Lara da, Campos, S?rgio Gaspar de
Jazyk: portugalština
Rok vydání: 2019
Předmět:
Zdroj: Biblioteca Digital de Teses e Dissertações da UFRRJ
Universidade Federal Rural do Rio de Janeiro (UFRRJ)
instacron:UFRRJ
Popis: Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2021-10-21T00:44:18Z No. of bitstreams: 1 2019 - M?rio Mendes Bonci.pdf: 2505225 bytes, checksum: 13dbb06949588f0e084c7287bb2e8d4c (MD5) Made available in DSpace on 2021-10-21T00:44:18Z (GMT). No. of bitstreams: 1 2019 - M?rio Mendes Bonci.pdf: 2505225 bytes, checksum: 13dbb06949588f0e084c7287bb2e8d4c (MD5) Previous issue date: 2019-04-26 CAPES - Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior Dermatophytes are a fungi group that require keratin for growth, colonizing the skin and appendages of animals and people. Although not obligatory pathogens, Microsporum gypseum, M. canis, and Trichophyton spp. are related to infections in animals, usually associated with circular lesions on the skin. Dermatophytosis by geophilic dermatophytes occurs to a lesser extent, having M. gypseum as the main etiologic agent involved. Among the factors linked to the ability of dermatophytes to infect animals, we highlight enzymes such as DNase, gelatinase, lipase, keratinase, elastase, and collagenase, which were evaluated in the present study. The behavior of domestic animals to be in close contact with the ground, in backyards, public roads, and parks justify the study of geophilic dermatophytes virulence factors and the changes on the enzymatic production between clinical and environmental isolates, which may contribute to control strategies and prevention of dermatophytosis. The main goal of this study was to evaluate the production capacity of enzymes related to the pathogenicity of dermatophytes (DNase, lipase, gelatinase, keratinase, elastase, and collagenase), among environmental and clinical isolates. The environmental strains were obtained by the technique described by Vanbreuseghem (1952), which uses pre-sterilized equines hair mixed with the moist soil, using soil samples from different Brazilian locations. The clinical specimens were obtained from animal hairs and crusts sent to the Veterinary Microbiological Diagnostic Service / UFRRJ and isolated in Mycosel? medium. The enzymatic evaluation of the dermatophytes was performed by spectrophotometry absorbance (collagenase, elastase, and keratinase), enzymatic degradation in Petri dishes (DNase and lipase) and liquefaction in tubes (gelatinase). In a total of 30 clinical isolates, M. canis (11), M. gypseum (7), M. nanum (2), T. mentagrophytes (4) and Trichophyton sp. (6), were identified. Among the 30 environmental isolates are M. gypseum (25), M. nanum (1) and Trichophyton sp. (4). There was no statistically significant difference in the production of keratinase, elastase, lipase, and gelatinase between the clinical and environmental groups, with the synthesis of all the enzymes for most evaluated isolates. There was a statistically significant difference between the groups in the production of collagenase and DNase, with emphasis on the absence of DNase production by most of the environmental isolates. Thus, dermatophytes from clinical and soil samples are able to produce keratinase, elastase, collagenase, DNase, lipase and gelatinase, enzymes related to different stages of the dermatophytic infection in animals and humans. Os dermat?fitos constituem grupo de fungos que requerem queratina para crescimento, colonizando a pele e anexos de animais e pessoas. Embora n?o sejam pat?genos obrigat?rios, Microsporum gypseum, M. canis e Trichophyton spp. relacionam-se a infec??es em animais, causando classicamente les?es circulares na pele. Embora as dermatofitoses por dermat?fitos geof?licos ocorram em menor grau, M. gypseum ? a principal esp?cie envolvida e estes quadros devem ser considerados. Dentre os fatores que fazem com que os dermat?fitos, tenham capacidade de infec??o para animais, destacam-se enzimas como DNase, gelatinase, lipase, queratinase, elastase e colagenase, que foram avaliadas neste trabalho. O h?bito dos animais dom?sticos permanecerem em contato ?ntimo com a terra, em quintais, vias p?blicas e parques, justifica o estudo de sua patogenicidade e a diferencia??o do perfil enzim?tico de isolados cl?nicos e ambientais de dermat?fitos, o que pode colaborar para estrat?gias de controle e preven??o da dermatofitose. Nossa pesquisa teve como objetivos o isolamento cl?nico e ambiental de fungos dermat?fitos, avaliando a produ??o de DNase, lipase, gelatinase, queratinase, elastase e colagenase, enzimas relacionadas ? virul?ncia dos mesmos e a compara??o da capacidade de produ??o dessas enzimas relacionadas ? patogenicidade entre as cepas cl?nicas e ambientais. As cepas ambientais foram obtidas atrav?s da t?cnica descrita por Vanbreuseghem (1952), que emprega pelos est?reis de equinos misturados ao solo umedecido, sendo utilizadas amostras de solo oriundas de diferentes locais do Brasil. As amostras cl?nicas foram oriundas de pelos e crostas de animais enviadas ao Servi?o de Diagn?stico Microbiol?gico Veterin?rio/UFRRJ e semeadas em meio Mycosel?. A avalia??o enzim?tica dos dermat?fitos foi feita por leituras de absorb?ncia em espectrofot?metro (colagenase, elastase e queratinase), forma??o de halo de degrada??o em placas (DNase e lipase) e liquefa??o em tubos (gelatinase). Dentre os 30 isolados cl?nicos est?o M. canis (11), M. gypseum (7), M. nanum (2), T. mentagrophytes (4) e Trichophyton sp. (6). e dentre os 30 isolados ambientais est?o M. gypseum (25), M. nanum (1) e Trichophyton sp. (4). N?o houve diferen?a significativa estatisticamente na produ??o de queratinase, elastase, lipase e gelatinase entre os grupos de isolados cl?nicos e ambientais, sendo a maior parte dos fungos produtora de todas estas enzimas. Houve diferen?a significativa estatisticamente entre os grupos na produ??o de colagenase e DNase, com destaque para a aus?ncia de produ??o de DNase pela maioria dos isolados ambientais. Assim, conclui-se que os dermat?fitos oriundos de amostras cl?nicas e do solo s?o capazes de produzir queratinase, elastase, colagenase, DNase, lipase e gelatinase, enzimas relacionas a diferentes etapas do processo da infec??o dermatof?tica em animais e pessoas.
Databáze: OpenAIRE
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