Effect of complexed and uncomplexed α-phelandrene in Hydroxypropyl-β-Cyclodextrin on orofacial nociception in rodents
| Autor: | Machado, Brennda Gonzaga |
|---|---|
| Přispěvatelé: | Antoniolli, Ângelo Roberto, Quintans, Jullyana de Souza Siqueira |
| Jazyk: | portugalština |
| Rok vydání: | 2022 |
| Předmět: | |
| Zdroj: | Repositório Institucional da UFS Universidade Federal de Sergipe (UFS) instacron:UFS |
| Popis: | Objective: the work aims to expand the studies involving the pharmacological effects of the αphelandrene monoterpene, specifically in the promotion of orofacial antinociception. Methods: a hydroxypropil-β-cyclodextrin (HPβ-CD) complex was prepared with incorporation of the α-felandorene that was later physically characterized through high efficiency liquid chromatography (CLAE) and differential exploratory calorimetry (DSC). Seeking to evaluate the possible analgesic action of α-felandreno in animal models in orofacial nociception, the animals were divided into four groups (n = 8 per group) and treated with unlapled α-felandreno (FEN) at the 50mg/kg oral dose , α-felandreno complex in hydroxipropil-β-cyclodextrin (FENHPβCD) at a dose of 50mg/kg oral via, negative control (tween 80 0.2% in water) 10ml/kg per gavage and positive controls (morphine 10mg/kg i.p. For tests of formaline, glutamate and hypertonic saline; camphor 7.6mg/kg i.p. for the scine test and, diazepam 3mg/kg i.p. for the rod rod test). Finally, quantification of TNF-α and IL-1β cytokines was made in the Vibrissa region after the formaline test and animal locomotor analysis analysis through the motor coordination test and data collection for statistical evaluations. Results: in the α-felandorereno quantification test by Clae, a 5.8 minutes retention time was found and a peak of good chromatographic resolution, the correlation coefficient R2 = 0.9998 proves the linearity of the method and the limit results Detection (LD) and quantification (LQ) (0.81 and 2.44 µg/mL, respectively) demonstrated the sensitivity of the method. DSC results indicated greater α-felandreno stability when incorporated into HPβ-CD. In formalin-induced nociception, a significant antinociceptive effect was observed in animals treated with FEN and FEN-HPβCD 50mg/kg when compared with the vehicle in both the neurogenic (p |
| Databáze: | OpenAIRE |
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