Effect of intracellular thirst on exploratory and anxiety-like responses in rats: possible participation of AVPR1a and OTR receptors

Autor: Souza, Viviane Felintro de
Přispěvatelé: Mecawi, Andr? de Souza, Rocha, F?bio Fagundes da, Borges, Danilo Lustrino
Jazyk: portugalština
Rok vydání: 2018
Předmět:
Zdroj: Biblioteca Digital de Teses e Dissertações da UFRRJ
Universidade Federal Rural do Rio de Janeiro (UFRRJ)
instacron:UFRRJ
Popis: Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2021-09-12T23:36:25Z No. of bitstreams: 1 2018 - Viviane Felintro de Souza.pdf: 1710801 bytes, checksum: ff3d98a5cdc76bfc7cd0479e45fe5a6e (MD5) Made available in DSpace on 2021-09-12T23:36:25Z (GMT). No. of bitstreams: 1 2018 - Viviane Felintro de Souza.pdf: 1710801 bytes, checksum: ff3d98a5cdc76bfc7cd0479e45fe5a6e (MD5) Previous issue date: 2018-03-09 Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior, CAPES, Brasil. Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico, CNPq, Brasil. Funda??o Carlos Chagas Filho de Amparo ? Pesquisa do Estado do Rio de Janeiro, FAPERJ, Brasil. Thirst is a sensation that induces water intake. This behavior is essential for the maintenance of life, and the vertebrates resort to a set of neurohumoral mechanisms that constantly regulate the body water and sodium content. Different osmotic stimuli, such as water deprivation and salt loading, trigger the release of vasopressin (AVP) and oxytocin (OT) and, consequently, their systemic actions. Recent studies have demonstrated that magnocellular vasopressinergic paraventricular neurons project to extrahypothalamic regions, which include the lateral habenula and central amygdala. Thus, it is possible that these structures and neuropeptides are involved with the changes in anxiety-like/motivated behaviors under conditions of hyperosmolality. The objective of this study was to analyze the effect of the hyperosmolality induced by dehydration on anxiety-like behaviors and gene expression of the AVP and OT receptorsas well as, the enzyme glutamate decarboxylase (GAD) and the vesicular glutamate transporter (vGLUT) in amygdala and habenula. For this purpose, we used Wistar rats (~300g) separated into five different groups: control (CT), water deprivation for 24 hours (WD24) and 48hours (WD48) and salt loading wich NaCl 1.8% for 24 hours (SL24) and 48 hours (SL48). These groups were submitted to the open field test (OF) followed by the elevated plus maze (EPM) while another set of rats underwent the light/dark box test (LDB). All tests were carried out in the night period. To verify the influence of dehydration in gene expression we used RT-qPCR. All data are described as mean ? standard error of the mean and statistical significance was set at p
Databáze: OpenAIRE
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