Cryprotectant effect of trehalose and low-density lipoprotein in extenders for frozen ram sperm
| Autor: | Tonieto, Rafael Adolfo, Goulart, Karina Lemos, Gastal, Gustavo Desire Antunes, Schiavon, Raquel Schiavon, Deschamps, João Carlos, Lucia Júnior, Thomaz |
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| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: | |
| Zdroj: | Repositório Institucional da UFPEL Universidade Federal de Pelotas (UFPEL) instacron:UFPEL |
| Popis: | Submitted by Maria Beatriz Vieira (mbeatriz.vieira@gmail.com) on 2010-10-08T13:37:47Z No. of bitstreams: 1 SmallRumRes - Tonieto et al 2010 - Threalose and LDL on extenders for frozen ram semen.pdf: 285880 bytes, checksum: b8425358e42c1489ad482ced83617fe4 (MD5) Made available in DSpace on 2010-10-08T13:37:47Z (GMT). No. of bitstreams: 1 SmallRumRes - Tonieto et al 2010 - Threalose and LDL on extenders for frozen ram semen.pdf: 285880 bytes, checksum: b8425358e42c1489ad482ced83617fe4 (MD5) Previous issue date: 2010-06-09 This study tested trehalose and low-density lipoprotein (LDL) as cryoprotectants in extenders for frozen ram semen. In the first experiment, the extenders were Tris, with 20% egg yolk (E1-1); E1-1 with 5% glycerol (E1-2); E1-1 with 100mM trehalose (E1-3); and E1-1 with 100mM trehalose and 5% glycerol (E1-4). Sperm motility and membrane integrity of the E1-2, E1-3 and E1-4 extenders were greater than for E1-1 (P < 0.05), but acrosome integrity following cryopreservation did not differ. In the second experiment, the extenders were Tris, with 20% egg yolk and 100mMtrehalose (E2-1); Tris with 8% LDL and 5% glycerol(E2-2); Tris with 8% LDL and 100mM trehalose (E2-3); and Tris with 8% LDL, 100mM trehalose and 5% glycerol (E2-4). Sperm membrane integrity was lowest for the E2-1 extender (P < 0.05), but similar for extenders including LDL. Sperm motility post-thawing was highest for E2-2 and E2-3 extenders (P < 0.05), but acrosome integrity did not differ. Thus, extenders including trehalose and LDL as cryoprotectants recorded a post-thawing ram sperm quality similar to that achieved when using conventional cryoprotectants. |
| Databáze: | OpenAIRE |
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