Single exercise stress reduces central neurotrophins levels and adenosine A1 and A2 receptors expression, but does not revert opioid-induced hyperalgesia in rats
| Autor: | Medeiros, Liciane Fernandes, Nunes, Éllen Almeida, Lopes, Bettega Costa, Souza, Andressa de, Cappellari, Angélica Regina, Freitas, Joice Soares de, Macedo, Isabel Cristina de, Kuo, Jonnsin, Cioato, Stefania Giotti, Battastini, Ana Maria de Oliveira, Caumo, Wolnei, Torres, Iraci L. S. |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: | |
| Zdroj: | Repositório Institucional do Centro Universitário La Salle Universidade La Salle (UNILASALLE) instacron:UNILASALLE |
| Popis: | Submitted by DSpace Unilasalle (dspace@unilasalle.edu.br) on 2021-08-02T18:59:56Z No. of bitstreams: 1 lfmedeiros.etal.pdf: 929739 bytes, checksum: ce360721638f449ca416ded050bd121b (MD5) Made available in DSpace on 2021-08-02T18:59:56Z (GMT). No. of bitstreams: 1 lfmedeiros.etal.pdf: 929739 bytes, checksum: ce360721638f449ca416ded050bd121b (MD5) Previous issue date: 2020 Background: This study assessed the effects of an acute stress model upon the long-term hyperalgesia induced by repeated morphine administration in neonatal rats. We also evaluated neurotrophins and cytokines levels; expressions of adenosine and acetylcholine receptors, and acetylcholinesterase enzyme at the spinal cord. Material and methods: Male Wistar rats were subjected to morphine or saline administration from P8 to P14. Thermal hyperalgesia and mechanical hyperesthesia were assessed using the hot plate (HP) and von Frey (vF) tests, respectively, at postnatal day P30 and P60. After baseline measurements, rats were subjected to a single exercise session, as an acute stress model, at P30 or P60. We measured the levels of BDNF and NGF, interleukin-6, and IL-10 in the cerebral cortex and the brainstem; and the expression levels of adenosine and muscarinic receptors, as well as acetylcholinesterase (AChE) enzyme at the spinal cord. Results: A stress exercise session was not able to revert the morphine-induced hyperalgesia. The morphine and exercise association in rats induced a decrease in the neurotrophins brainstem levels, and A1 , A2A , A2B receptors expression in the spinal cord, and an increase in the IL-6 cortical levels. The exercise reduced M2 receptors expression in the spinal cord of naive rats, while morphine prevented this effect. Conclusions: Single session of exercise does not revert hyperalgesia induced by morphine in rats; however, morphine plus exercise modulate neurotrophins, IL-6 central levels, and expression of adenosine receptors. |
| Databáze: | OpenAIRE |
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