Astrocyte intermediate filaments are important markers in olfactory bulb of bovine herpesvirus type 5 natural infections

Autor: Gomes, Deriane E. [UNESP], Ferrari, Heitor F. [UNESP], Roncatti, Flávio T.L.B. [UNESP], Paes, Fernanda [UNESP], Cardoso, Lívia S. [UNESP], Perri, Silvia Helena Venturoli [UNESP], Nogueira, Adriana H. C., Luvizotto, Maria Cecília R. [UNESP], Cardoso, Tereza C. [UNESP]
Přispěvatelé: Universidade Estadual Paulista (Unesp), Instituto Biológico
Jazyk: angličtina
Rok vydání: 2010
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Zdroj: Scopus
Repositório Institucional da UNESP
Universidade Estadual Paulista (UNESP)
instacron:UNESP
Popis: Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-27T11:24:41Z No. of bitstreams: 0 Made available in DSpace on 2014-05-27T11:24:41Z (GMT). No. of bitstreams: 0 Previous issue date: 2010-05-01 Astroglial cells are the most abundant cells in the mammalian central nervous system, yet our knowledge about their function in bovine Herpesvirus type 5 (BoHV-5) has been limited. The aim of this study was to detect by immunohistochemistry assay the reactive astrocytes for glial fibrilary acidic protein (GFAP) and vimentin (VIM), considered intermediate filaments of the cytoskeleton, localized in olfactory bulb from natural acute cases of BoHV-5 infection. All samples were submitted to virus isolation, real-time polymerase chain reaction (RT-PCR) and in situ hybridization (ISH) technique to confirm the virus transcription and respective genome. Samples were classified into four groups according to the severity of histological lesions. Groups III and IV, which histological lesions were classified as alacia, gliosis, satellitosis, neuronophagia and neuronal necrosis, 35% (± 1.8-2.1) of the inflammatory mononuclear cells, corresponded to CD3 positive lymphocytes. In the same group, 35% (± 1.8) of astrocytes were described as reactive to GFAP and VIM proteins. An agreement of r = 1.0 (P
Databáze: OpenAIRE
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