Brazilian Archives of Biology and Technology
| Autor: | Barreto, George Emílio Sampaio, Oliveira, Elineusa Silva de, Villar, Flávio Augusto Guerreiro Aragão de, Santos, Gleide Souza dos, Lima, Rute Maria Ferreira, Costa, Silvia Lima, Costa, Maria de Fátima Dias, Egito, Eryvaldo Sócrates Tabosa, El-Bachá, Ramon dos Santos |
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| Jazyk: | angličtina |
| Rok vydání: | 2007 |
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| Zdroj: | Repositório Institucional da UFBA Universidade Federal da Bahia (UFBA) instacron:UFBA |
| DOI: | 10.1590/S1516-89132007000500012 |
| Popis: | p.839-849 Submitted by Texeira Ana (atanateixeira@gmail.com) on 2012-09-25T17:47:22Z No. of bitstreams: 1 Barreto, George Emílio Sampaio.pdf: 114060 bytes, checksum: dd744d7abf1fcacb7bcd3775cd9d799d (MD5) Made available in DSpace on 2012-09-25T17:47:22Z (GMT). No. of bitstreams: 1 Barreto, George Emílio Sampaio.pdf: 114060 bytes, checksum: dd744d7abf1fcacb7bcd3775cd9d799d (MD5) Previous issue date: 2007-09 The aim of this work was to study the effects of 3MC on the peroxidation of biomolecules in nuclear fractions and nonsynaptic mitochondrial respiration in organelles obtained from rat brains. The cytotoxicity towards rat primary astrocytes in vitro was also tested. 3MC at 1mM oxidized consuming oxygen at a rate of 1.98 ± 0.19 µM.min-1 and formed reactive quinones. At the same concentration, 3MC induced peroxidation of biomolecules in nuclear fractions obtained from rat brain homogenates and inhibited state 2 FADH2-linked respiration in nonsynaptic mitochondria. Furthermore, 3MC oxidized in the culture medium, leading to the formation of quinones. This toluene metabolite was cytotoxic to rat primary astrocytes. The concentration that killed 50% of cells after 72 h was 107 mM. The results of the study indicated a direct relationship between cytotoxicity and 3MC oxidation. Curitiba |
| Databáze: | OpenAIRE |
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