PARADOXICAL EFFECT OF QUERCETIN ANTIOXIDANT ON GOAT SPERM PARAMETERS AFTER CRYOPRESERVATION
| Autor: | Borges, Marcelo Sant'Ana, Barbosa Born, Joana Larissa, Conti, Leticia Martins, Segabinazzi, Lorenzo Garrido [UNESP], Nichi, Marcilio, Vechiato Kawai, Giulia Kiyomi, Leite, Roberta Ferreira, Peixoto, Kleber da Cunha, Dell'Aqua, Jose Antonio [UNESP], Papa, Frederico Ozanam [UNESP], Crespilho, Andre Maciel |
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| Přispěvatelé: | Univ Santo Amaro, Universidade Estadual Paulista (Unesp), Universidade de São Paulo (USP) |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: | |
| Zdroj: | Web of Science Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| Popis: | Made available in DSpace on 2020-12-10T20:14:02Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-05-01 BACKGROUND: Some antioxidants have been used in semen extenders to reduce adverse effects caused by excessive reactive oxygen species (ROS) production. The study was carried out to assess the effect of quercetin (QC) antioxidant therapy on goat semen submitted to cryopreservation. OBJECTIVE: To evaluate the effect of quercetin incorporation in different phases of the cryopreservation process of goat spermatozoa. MATERIALS AND METHODS: Five ejaculates from each of four goats (n=20) were collected and split into four groups: Control (G1), without QC; G2, 15 mu M of QC added to semen before centrifugation; G3, 15 mu M QC added to semen after centrifugation; G4, 15 mu M QC added to semen before centrifugation and 15 mu M of QC added to semen after centrifugation (total of 30 mu M of QC); and cryopreserved. All semen samples were evaluated after thawing for sperm kinetics, plasma membrane integrity, and ROS levels. RESULTS: Although lower concentrations of ROS were associated with groups that received antioxidant supplementation (P=0.0213), linear and dose dependent (P |
| Databáze: | OpenAIRE |
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