Acta Tropica
| Autor: | Silva, Evandro Moraes, Antunes, Fabiana Rodrigues, Rodrigues, Marcio Silva, Julião, Fred da Silva, Lima, Artur Gomes Dias, Sousa, Valderez Lemos de, Alcântara, Adriano Costa de, Reis, Eliana Almeida Gomes, Nakatani, Maria, Badaró, Roberto José da Silva, Reis, Mitermayer Galvão, Carvalho, Lain Carlos Pontes de, Franke, Carlos Roberto |
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| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: | |
| Zdroj: | Repositório Institucional da UFBA Universidade Federal da Bahia (UFBA) instacron:UFBA |
| DOI: | 10.1016/j.actatropica.2006.04.002 |
| Popis: | RESTRITO Submitted by Santiago Fabio (fabio.ssantiago@hotmail.com) on 2012-07-31T19:53:28Z No. of bitstreams: 1 HH.pdf: 201900 bytes, checksum: 640cdc682dabd16c562ec2fca3832784 (MD5) Made available in DSpace on 2012-07-31T19:53:28Z (GMT). No. of bitstreams: 1 HH.pdf: 201900 bytes, checksum: 640cdc682dabd16c562ec2fca3832784 (MD5) Previous issue date: 2006 In order to investigate whether pigs can be infected by Leishmania infantum, a serological and parasitological study was carried out on swine in the Jequié municipality, Northeast of Brazil. Anti-Leishmania infantum antibodies were detected in 37 out of 92 swine (40.2%), by two different assays: an anti-L. infantum lysate and an anti-K39 recombinant protein ELISA. An experimental study was also carried out to verify the susceptibility of domestic pigs to L. infantum infection. Three sows inoculated with 108 stationary-phase infective L. infantum promastigotes (26% metacyclic promastigotes) per kilogram of body weight produced anti-Leishmania antibodies until the end of the experiment, 11 months later. No parasites, however, could be visualized through optical microscopy of spleen, liver and bone marrow or by in vitro culture of these organs. Homogenates of these organs were also inoculated in hamsters, without producing infection. No Leishmania DNA was detected by polymerase chain reaction (PCR) in sand flies fed on these animals. The results indicate that domestic pigs bitten by L. infantum-infected vectors in the endemic area do not display a full infection pattern, and the positive association in endemic areas between the presence of swine and infection in canines may not be ascribable to the former acting as a parasite reservoir. Salvador |
| Databáze: | OpenAIRE |
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