Hybridoma and Hybridomics
| Autor: | Santos, Roberto Robson Borges dos, Larangeira, Daniela Farias, Aguiar, Paulo Henrique Palis, Santos, Marta Almeida dos, Melo, Stella Maria Barrouin, Silva, Tânia Maria Correia, Mengel, José O., Santos, Washington Luis Conrado dos, Carvalho, Lain Carlos Pontes de |
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| Jazyk: | angličtina |
| Rok vydání: | 2004 |
| Zdroj: | Repositório Institucional da UFBA Universidade Federal da Bahia (UFBA) instacron:UFBA |
| DOI: | 10.1089/1536859041651303 |
| Popis: | Texto completo: acesso restrito. p. 250-257 Submitted by Edileide Reis (leyde-landy@hotmail.com) on 2013-12-13T13:22:30Z No. of bitstreams: 1 Roberto Robson Borges dos Santos.pdf: 5019692 bytes, checksum: 9825d1d27bddf5c09253a29d1edb934c (MD5) Approved for entry into archive by Alda Lima da Silva (sivalda@ufba.br) on 2014-02-13T18:35:31Z (GMT) No. of bitstreams: 1 Roberto Robson Borges dos Santos.pdf: 5019692 bytes, checksum: 9825d1d27bddf5c09253a29d1edb934c (MD5) Made available in DSpace on 2014-02-13T18:35:31Z (GMT). No. of bitstreams: 1 Roberto Robson Borges dos Santos.pdf: 5019692 bytes, checksum: 9825d1d27bddf5c09253a29d1edb934c (MD5) Previous issue date: 2004 The production and partial characterization of a monoclonal antibody, the IgG1 IH1, which recognizes an antigen distributed in canine monocytes/macrophages, is reported here. The distribution and apparent molecular weight of the antigen recognized by the IH1 MAb was determined in peripheral blood leukocytes, peripheral blood monocyte-derived macrophages and tissue sections of spleen, liver and skin, using Western blotting, immunocytochemistry, immunohistochemistry and flow cytometry. The IH1 MAb-recognized antigen was detected in Western blotting under non-reducing conditions spread out as a large band covering the position corresponding to the migration of molecules with molecular weights from 55 to 73 kDa. The IH1 MAb labeled blood monocytes, tissue macrophages in lymph nodes, and in the mantle zone of the spleen, and Kupffer cells in the liver. It did not react with human cells. In flow cytometric analysis, the IH1 MAb reacted with a subpopulation of monocytes. The MAb described herein may become a valuable tool for diagnosis and research on canine diseases. |
| Databáze: | OpenAIRE |
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