Characterization of Plasmodium falciparum strains of the State Rond?nia Brazil, using microtests sensitivity to antimalarials microtechniques, enzymatic typing and monoclonal antibodies
| Autor: | Di Santi, Silvia Maria, Boulos, Marcos, Vasconcelos, M. A, Oliveira, Salma Gomes de, Couto, A, Rosario, Virgilio Estolio do |
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| Jazyk: | portugalština |
| Rok vydání: | 1987 |
| Předmět: | |
| Zdroj: | Repositório Digital do Instituto Evandro Chagas (Patuá) Instituto Evandro Chagas (IEC) instacron:IEC |
| Popis: | Secretaria de Estado da Sa?de. Superintend?ncia de Controle de Endemias. Laborat?rio de Mal?ria. S?o Paulo, SP, Brasil. Secretaria de Estado da Sa?de. Superintend?ncia de Controle de Endemias. Laborat?rio de Mal?ria. S?o Paulo, SP, Brasil. Minist?rio da Sa?de. Funda??o Servi?os de Sa?de P?blica. Instituto Evandro Chagas. Bel?m, PA, Brasil. Minist?rio da Sa?de. Funda??o Servi?os de Sa?de P?blica. Instituto Evandro Chagas. Bel?m, PA, Brasil. Minist?rio da Sa?de. Funda??o Servi?os de Sa?de P?blica. Instituto Evandro Chagas. Bel?m, PA, Brasil. Blomedlcal Research Institute. Maryland, USA. Nove amostras de Plasmodium falciparum foram coletadas de migrantes infectados no Estado de Rond?nia. Estas amostras foram mantidas em cultivo para caracteriza??o por tipifica??o por enzim?tica em acetato de celulose, sensibilidade a cloroquina, amodiaquina, mefloquina e quinino e an?lise da diversidade antig?nica atrav?s de anticorpos monoclonais espec?ficos. Os resultados obtidos mostraram varia??o entre todas as amostras estudadas: encontrou-se resist?ncia crescente a cloroquina, resist?ncia intermediaria a amodiaquina e quinino e sensibilidade a baixos n?veis de mefloquina; apenas dois isolados mostraram sensibilidade a todas as drogas. A tipifica??o enzim?tica mostrou presen?a de parasitas GPI 1 e 2 e ADA 1 e 2, enquanto que para PEP e LDH todas as amostras foram do tipo 1. Sorotipagem com anticorpos monoclonais PSA mostrou presencia de tr?s sorotipos diferentes (II, III E IV). Estes resultados mostraram: a) varia??o entre as amostras para os marcadores analisados; b) nesta regi?o, para o pequeno numero de amostras analisadas, n?o foram observadas diferen?as significativas ou novos tipos de parasitas. Nine samples of Plasmodium falciparum were collected from migrant workers who were infected in the State of Rond?nia, Brazil. These samples were maintained in culture for characterlzation by enzyme typing using acetate cellulose electrophoresis, drug susceptlbility to chioroquine, amodiaquine, mefloquine and quinnine and antigenic diversity analysis using strain specific monoclonal antibodies. The results obtained have confirmed major variation among alI samples studied: it was found consistent resistance to chloroquine moderated resistance to amodiaquine and quinine and susceptibllity to low levels of mefloquine; only two isolates have shown susceptibility to alI drugs in the in vitre assays. Enzyme typing showed presence of both GPI 1 and 2 and ADA 1 and 2 parasites, while for PEP and LDR alI samples were type 1. Serotyping with PSA monoclonal antibodies show. ed presence of three different serotypes (lI, III and IV). These results confirm: a) variation among samples for markers analysed; b) In this area and for the small number of samples studied, no significant differences or new types of parasites were seen. |
| Databáze: | OpenAIRE |
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