Exig??ncia de lisina para juvenis de Tambaqui (Colossoma macropomum CUVIER, 1818) com base no desempenho produtivo, morfohistol??gico e fisiol??gico

Autor: Liebl, Ariany Rabello da Silva
Přispěvatelé: Oliveira, Adriano Teixeira de, Furuya, Wilson Massamitu, Bussons, M??rcia Regina Fragoso Machado, Oliveira, Ana Cristina Belarmino de, Oliveira, Alzira Miranda de, Jesus, Rog??rio Souza de, Aride, Paulo Henrique Rocha
Jazyk: portugalština
Rok vydání: 2019
Předmět:
Zdroj: Biblioteca Digital de Teses e Dissertações da UFAM
Universidade Federal do Amazonas (UFAM)
instacron:UFAM
Popis: Submitted by Ariany Liebl (ny.rabello@gmail.com) on 2019-11-02T00:52:47Z No. of bitstreams: 3 TESE CIPET - UFAM - LIEBL 2019 - FINAL.pdf: 2652511 bytes, checksum: 1a0725fc5a7060db397532e7f6a2a5a6 (MD5) Carta Encaminhamento Autodep??sito.pdf: 88882 bytes, checksum: cb83e79b7a0901b9a6f6e4cd3ab5f95b (MD5) Folha de aprova????o.pdf: 590738 bytes, checksum: b933bbebb9d628e86eb5b32245434387 (MD5) Approved for entry into archive by Ci??ncias Pesqueiras nos Tr??picos PPGCIPET (ppgcipet@ufam.edu.br) on 2019-11-04T17:50:10Z (GMT) No. of bitstreams: 3 TESE CIPET - UFAM - LIEBL 2019 - FINAL.pdf: 2652511 bytes, checksum: 1a0725fc5a7060db397532e7f6a2a5a6 (MD5) Carta Encaminhamento Autodep??sito.pdf: 88882 bytes, checksum: cb83e79b7a0901b9a6f6e4cd3ab5f95b (MD5) Folha de aprova????o.pdf: 590738 bytes, checksum: b933bbebb9d628e86eb5b32245434387 (MD5) Approved for entry into archive by Ci??ncias Pesqueiras nos Tr??picos PPGCIPET (ppgcipet@ufam.edu.br) on 2019-11-04T17:50:10Z (GMT) No. of bitstreams: 3 TESE CIPET - UFAM - LIEBL 2019 - FINAL.pdf: 2652511 bytes, checksum: 1a0725fc5a7060db397532e7f6a2a5a6 (MD5) Carta Encaminhamento Autodep??sito.pdf: 88882 bytes, checksum: cb83e79b7a0901b9a6f6e4cd3ab5f95b (MD5) Folha de aprova????o.pdf: 590738 bytes, checksum: b933bbebb9d628e86eb5b32245434387 (MD5) Approved for entry into archive by Divis??o de Documenta????o/BC Biblioteca Central (ddbc@ufam.edu.br) on 2019-11-04T20:27:54Z (GMT) No. of bitstreams: 3 TESE CIPET - UFAM - LIEBL 2019 - FINAL.pdf: 2652511 bytes, checksum: 1a0725fc5a7060db397532e7f6a2a5a6 (MD5) Carta Encaminhamento Autodep??sito.pdf: 88882 bytes, checksum: cb83e79b7a0901b9a6f6e4cd3ab5f95b (MD5) Folha de aprova????o.pdf: 590738 bytes, checksum: b933bbebb9d628e86eb5b32245434387 (MD5) Made available in DSpace on 2019-11-04T20:27:54Z (GMT). No. of bitstreams: 3 TESE CIPET - UFAM - LIEBL 2019 - FINAL.pdf: 2652511 bytes, checksum: 1a0725fc5a7060db397532e7f6a2a5a6 (MD5) Carta Encaminhamento Autodep??sito.pdf: 88882 bytes, checksum: cb83e79b7a0901b9a6f6e4cd3ab5f95b (MD5) Folha de aprova????o.pdf: 590738 bytes, checksum: b933bbebb9d628e86eb5b32245434387 (MD5) Previous issue date: 2019-09-30 CAPES - Coordena????o de Aperfei??oamento de Pessoal de N??vel Superior 92981977541 The level of lysine requirement can be determined by methods such as dose response associated with the ideal protein concept, and tends to improve yield performance while maintaining fish health. The objective of this thesis was to determine the lysine requirement for maximum performance of tambaqui (Colossoma macropomum) juveniles, concomitantly, to verify the influence of amino acid on blood count and blood biochemistry, activity of digestive enzymes, intestinal and hepatic histomorphology, fillet chemical composition and yield trends of fish. The variables analyzed were validated by ANOVA, Tukey's test, and regression analysis (p0.05). Furthermore, no differences were found for hemoglobin, hematocrit, MCHC, eosinophil, monocyte, thrombocyte, glucose, triglyceride concentrations for amylase activity, lipase, and morphometric variables of the fish body (p>0.05). However, final weight (p=0.01), relative growth rate - length (p = 0.02), total weight gain (p=0.04), lysine efficiency (p=0.00 ) and apparent feed conversion (p = 0.00) differed at the level of 15.96 g. Kg-1 of total lysine. Lysine consumption (p = 0.00) and fin length (p=0.00) of tambourines linearly increased lysine levels. Intestinal tunics in the anterior muscle (p=0.00), anterior submucosa (p=0.00) and middle (p=0.01), and anterior mucosa (p=0.00) portions were influenced by diet as well as such as liver tissue, where hepatodystrophies were observed and increased hepatosomal index (p=0.04). The height of the midgut villi (p =0.01) was higher at the lowest L-lysine addition level (9.72 g.Kg-1 total lysine, 4.00 g L-lysine) and the perimeter level 12.84 g.Kg-1 of lysine overlapped with that of 22.20 g.Kg-1 of lysine. In the posterior intestine, height and perimeter (p=0.00; p=0.02) expressed variations not directly related to lysine levels. Acid mucins were higher in the anterior intestine at 22.20 g.Kg of lysine in the diet (p=0.001). MCV (p=0.00), MCH (p=0.00), total erythrocytes (p=0.02), lymphocytes (p=0.00) and neutrophils (p=0.00) were changed only in the initial period (p0,05). Outrossim, n??o foram constatadas diferen??as para as concentra????es de hemoglobina, hemat??crito, CHCM, eosin??filos, mon??citos, tromb??citos, glicose, triglicer??deos, para atividade da amilase, lipase, e para as vari??veis morfom??tricas do corpo do peixe (p>0,05). No entanto, o peso final (p=0,01), a taxa de crescimento relativo - comprimento (p=0,02), ganho de peso total (p=0,04), efici??ncia da lisina (p=0,00) e a convers??o alimentar aparente (p=0,00), diferiram ao n??vel 15, 96 g. Kg-1 de lisina total. O consumo da lisina (p=0,00) e o comprimento das nadadeiras (p=0,00) dos tambaquis aumentaram linearmente os n??veis de lisina. T??nicas intestinais nas por????es muscular anterior (p=0,00), submucosa anterior (p=0,00) e m??dia (p=0,01), e mucosa anterior (p=0,00), foram influenciadas pela dieta, bem como, o tecido hep??tico, onde foram verificadas hepatodistrofias e aumento do ??ndice hepatossom??tico (p=0,04). A altura das vilosidades do intestino m??dio (p=0,01) foi maior no n??vel de menor adi????o de L-lisina (9,72 g.Kg-1 de lisina total, 4,00 g de L-lisina) e o per??metro do n??vel 12,84 g.Kg-1 de lisina se sobrep??s ao do 22,20 g.Kg-1 de lisina. No intestino posterior, altura e per??metro (p=0,00; p=0,02) expressaram varia????es n??o relacionadas diretamente com os n??veis de lisina. Mucinas ??cidas foram mais elevadas no intestino anterior com 22,20 g. Kg-1 de lisina na dieta (p=0,001). O VCM (p=0,00), HCM (p=0,00), eritr??citos totais (p=0,02), linf??citos (p=0,00) e neutr??filos (p=0,00), foram alterados apenas no per??odo inicial (p
Databáze: OpenAIRE