Effects of photodynamic therapy in extracellular matriz of mouse lungs: an alternative tissue sterilization process

Autor: Apost??lico, Nadua
Přispěvatelé: Oliveira, Luis Vicente Franco de, Farr??, Ramon, Oliveira, Luis Vicente Franco, Vieira, Rodolfo de Paula, Prates, Renato Araujo, Deana, Alessandro Melo, Costa, Maricilia Silva
Jazyk: portugalština
Rok vydání: 2015
Předmět:
Zdroj: Biblioteca Digital de Teses e Dissertações da Uninove
Universidade Nove de Julho (UNINOVE)
instacron:UNINOVE
Popis: Submitted by Nadir Basilio (nadirsb@uninove.br) on 2018-06-19T15:58:48Z No. of bitstreams: 1 Nadua Apostolico.pdf: 2774487 bytes, checksum: c4de48bfb4f153970bee3d2f104e406e (MD5) Made available in DSpace on 2018-06-19T15:58:48Z (GMT). No. of bitstreams: 1 Nadua Apostolico.pdf: 2774487 bytes, checksum: c4de48bfb4f153970bee3d2f104e406e (MD5) Previous issue date: 2015-12-16 The sterilization step for acellular lungs prior to recellularization is important to ensure that they are free of any risk of transmitting infections from the donor to the recipient tissue/organ to be transplanted. However, there are no data on the mechanical effects induced in the lungs when subjected to a given dose of sterilization radiation through a 660-nm LED. The aim of this study was to evaluate the extracellular matrix (ECM) and lung mechanic parameters of decellularized lungs undergoing photodynamic therapy (PDT) with the purpose of sterilization, and also to perform microbiological analyses of lungs infected with C. albicans to determine the effectiveness of PDT. The lung mechanical evaluation parameters, Resistance (RL) and elastance (EL), exhibited no significant differences between groups. Also, there were no changes observed over time of irradiation, representing a maintenance of the viscoelastic behavior of the lung scaffold after 1h exposure to LED light. The ECM components remained virtually unchanged in the acellular lungs of both groups. We also showed that there was a reduction in fungi infection population after 45 minutes of PDT. However, more studies should be performed to establish and verify the effectiveness of PDT as a possible means for sterilizing lung scaffolds. A esteriliza????o de scaffolds pulmonares, antes de se iniciar a recelulariza????o, ?? um fator importante visando assegurar que pulm??es acelulares estejam livres de qualquer risco de transmiss??o de infec????es a partir do doador para o receptor do tecido / ??rg??o a ser transplantado. No entanto, n??o existem dados sobre os efeitos mec??nicos induzidos nestes pulm??es quando submetidos a terapia fotodin??mica. O objetivo deste estudo foi analisar a matriz extracelular (MEC) e avaliar par??metros de mec??nica pulmonar de pulm??es descelularizados submetidos ?? terapia fotodin??mica (TFD) como poss??vel esteriliza????o, e tamb??m realizar an??lises microbiol??gicas de pulm??es infectados por C. albicans para determinar a efetividade da TFD. Os valores obtidos para avalia????o do comportamento da mec??nica pulmonar, resist??ncia (RL) e elast??ncia (EL), n??o apresentaram diferen??as significativas entre os grupos. Al??m disso, n??o foram observadas altera????es ao longo do tempo de irradia????o, o que representa uma manuten????o do comportamento viscoel??stico dos scaffolds de pulm??o, ap??s 1h de exposi????o a luz LED. Os componentes da matriz extracelular permaneceram praticamente inalterados nos pulm??es acelulares de ambos os grupos. Tamb??m foi evidenciado que houve uma redu????o fungica ap??s 45 minutos de PDT. No entanto, mais estudos devem ser realizados para estabelecer e verificar a efic??cia do TFD como um poss??vel meio para esterilizar scaffolds pulmonares.
Databáze: OpenAIRE
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